Gelatinized copper-capillary alginate gel functions as an injectable tissue scaffolding system for stem cell transplants.
Gelatinized copper-capillary alginate gel functions as an injectable tissue scaffolding system for stem cell transplants.
复制标题
DOI：
10.1163/092050610x519453
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Weiss MD
中科院分区：
文献类型：
--
作者：
Willenberg BJ;Zheng T;Meng FW;Meneses JC;Rossignol C;Batich CD;Terada N;Steindler DA;Weiss MD
在严重的缺氧缺血性脑损伤中，细胞成分如神经元和星形胶质细胞与支持细胞外基质一起受到损伤或沿着破坏。这对再生医学领域提出了挑战，因为缺乏细胞外基质和支持结构使得移植环境对移植细胞不适宜。这个问题的一个潜在解决方案是使用一种生物材料来提供细胞外成分，以保持细胞位于囊性脑区域，使细胞形成连接并修复丢失的脑组织。理想情况下，这种生物材料将与干细胞结合，干细胞已被证明具有治疗潜力，并可以通过注射输送。为了研究这种方法，我们从低聚明胶和铜毛细管藻酸盐凝胶（GCCAG）中衍生出一种水凝胶生物材料组织支架。然后，我们证明了我们的多能星形胶质细胞干细胞（MASC）可以在体外GCCAG支架中维持长达2周，并且细胞保留了其多能性。我们接下来进行了一项试点移植研究，其中GCCAG与MASC混合并注射到新生大鼠幼崽的大脑中。在体内一周后，我们的结果表明：GCCAG生物材料没有引起显著的反应性胶质增生;活细胞保留在注射的支架内;并且一些递送的细胞迁移到周围的脑组织中。因此，GCCAG组织支架是一种很有前途的、新型的用于干细胞脑内移植的可注射系统。
In severe hypoxic–ischemic brain injury, cellular components such as neurons and astrocytes are injured or destroyed along with the supporting extracellular matrix. This presents a challenge to the field of regenerative medicine since the lack of extracellular matrix and supporting structures makes the transplant milieu inhospitable to the transplanted cells. A potential solution to this problem is the use of a biomaterial to provide the extracellular components needed to keep cells localized in cystic brain regions, allowing the cells to form connections and repair lost brain tissue. Ideally, this biomaterial would be combined with stem cells, which have been proven to have therapeutic potentials, and could be delivered via an injection. To study this approach, we derived a hydrogel biomaterial tissue scaffold from oligomeric gelatin and copper–capillary alginate gel (GCCAG). We then demonstrated that our multipotent astrocytic stem cells (MASCs) could be maintained in GCCAG scaffolds for up to 2 weeks in vitro and that the cells retained their multipotency. We next performed a pilot transplant study in which GCCAG was mixed with MASCs and injected into the brain of a neonatal rat pup. After a week in vivo, our results showed that: the GCCAG biomaterial did not cause a significant reactive gliosis; viable cells were retained within the injected scaffolds; and some delivered cells migrated into the surrounding brain tissue. Therefore, GCCAG tissue scaffolds are a promising, novel injectable system for transplantation of stem cells to the brain.