TERMINAL FIELD OF CHOLECYSTOKININ-8-LIKE IMMUNOREACTIVE PROJECTION NEURONS OF THE RAT MAIN OLFACTORY-BULB
TERMINAL FIELD OF CHOLECYSTOKININ-8-LIKE IMMUNOREACTIVE PROJECTION NEURONS OF THE RAT MAIN OLFACTORY-BULB
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DOI：
10.1002/cne.902850107
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发表时间：
1989-07-01
影响因子：
2.5
通讯作者：
TOHYAMA, M
中科院分区：
文献类型：
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作者：
MATSUTANI, S;SENBA, E;TOHYAMA, M
应用免疫组织化学方法，结合顺行示踪法和变性法，对大鼠主嗅球内胆囊收缩素-8（CCK）样免疫反应（CCK-IR）簇状细胞终末野进行了观察。将菜豆凝集素（PHA）注入动物的主嗅球，进行CCK免疫染色。对相邻切片分别进行CCK和PHA免疫染色。CCK-IR终末样染色在前嗅核Ia层和嗅结节外侧部可见较浓染色，在前嗅核与梨状皮质之间的过渡区、嗅结节内侧部和杏仁皮质核可见较弱染色。CCK-IR染色仅限于含有PHA标记的末端的区域，并且在单侧嗅球切除术后在这些部位减少。免疫电镜分析表明，CCK-IR公司在这些地区作出了不对称的突触接触，主要是与树突棘。这些结果表明，CCK-IR簇状细胞主要投射到嗅前核和嗅结节外侧部，并主要通过轴棘突触起作用。
The terminal field of cholecystokinin-8 (CCK)-like immunoreactive (CCK-IR) tufted cells in the rat main olfactory bulb was examined by means of immunohistochemistry combined with either an anterograde tracer or a degeneration method. CCK immunostaining was carried out in animals in which Phaseolus vulgaris agglutinin (PHA) had been injected into the main olfactory bulb. Pairs of adjacent sections were processed for CCK and PHA immunostaining, respectively. Dense CCK-IR terminallike staining was noted in layer Ia of the anterior olfactory nucleus and lateral part of the olfactory tubercle; weaker staining was also observed in the transitional area between the anterior olfactory nucleus and the piriform cortex, in the medial part of the olfactory tubercule, and in the cortical amygdaloid nucleus. The CCK-IR staining was limited to the area containing PHA-labeled terminals and was diminished in these sites after unilateral olfactory bulbectomy. Immuno-electron microscopic analysis showed that CCK-IR profiles in such regions made asymmetric synaptic contacts, mainly with dendritic spines. These results suggest that CCK-IR tufted cells project mainly to the anterior olfactory nucleus and lateral part of the olfactory tubercle, and act mainly via axospinous synapses.