A spider toxin, ω-agatoxin IV A, binds to fixed as well as living tissues: cytochemical visualization of P/Q-type calcium channels.
A spider toxin, ω-agatoxin IV A, binds to fixed as well as living tissues: cytochemical visualization of P/Q-type calcium channels.
复制标题
蜘蛛毒素 ω-agatoxin IV A 与固定组织和活组织结合：P/Q 型钙通道的细胞化学可视化。
作者：
S. Nakanishi
ω-Agatoxin IV A是一种来自Agelenopsis aperta毒液的肽基毒素，选择性地结合电压门控的P/ q型钙通道。ω-Agatoxin IV A已被用作药理学和电生理研究的选择性工具。利用生物素共轭ω-Agatoxin IV A在新鲜制备的小鼠小脑和海马切片中实现了P/ q型钙通道的可视化(Nakanishi等人，J. Neurosci。研究，41:532,1995)。本研究将生物素化的ω-agatoxin IV A应用于不同固定剂制备的经心固定脑片。ω-Agatoxin IV A不与P/ q型钙通道敲除小鼠的固定组织结合，证实与正常固定组织的结合不是人为的。在透射电镜下，金共轭链亲和素显示的生物素化ω-agatoxin IV A结合位点的位置与抗体显示的相似。生物素化ω-agatoxin IV A与固定组织结合的能力为研究突触分子结构提供了一种新的细胞化学技术。
ω-Agatoxin IV A, a peptidyl toxin from Agelenopsis aperta venom, selectively binds to voltage-gated P/Q-type calcium channels. ω-Agatoxin IV A has been used as a selective tool in pharmacological and electrophysiological studies. Visualization of P/Q-type calcium channels has previously been accomplished using biotin-conjugated ω-Agatoxin IV A in freshly prepared mouse cerebellar and hippocampal slices (Nakanishi et al, J. Neurosci. Res., 41: , 532, 1995). Here biotinylated ω-agatoxin IV A was applied to transcardially fixed brain slices prepared with various fixatives. ω-Agatoxin IV A did not bind to fixed tissues from P/Q-type calcium channel knockout mice, confirming that binding to normal, fixed tissues was not an artifact. Using transmission electron microscopy, locations of biotinylated ω-agatoxin IV A binding sites visualized with gold-conjugated streptavidin showed a similar pattern to those visualized with antibody. The ability of biotinylated ω-agatoxin IV A to bind to fixed tissue provides a new cytochemical technique to study molecular architecture of synapses.
影响因子：
5.3
作者：
Althof D;Baehrens D;Watanabe M;Suzuki N;Fakler B;Kulik Á
通讯作者：
Kulik Á