Quantitative Chemical Imaging of the Intracellular Spatial Distribution of Fundamental Elements and Light Metals in Single Cells
Quantitative Chemical Imaging of the Intracellular Spatial Distribution of Fundamental Elements and Light Metals in Single Cells
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DOI：
10.1021/ac5008909
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发表时间：
2014-05-20
影响因子：
7.4
通讯作者：
Lagomarsino, Stefano
中科院分区：
文献类型：
--
作者：
Malucelli, Emil;Iotti, Stefano;Lagomarsino, Stefano
我们报告了一种方法，可以对整个单细胞进行完整的定量表征，评估碳、氮、氧、钠和镁的总量，并提供元素摩尔浓度、细胞密度、质量和体积的亚微米图。这种方法允许量化低至 10(6) 个原子/μ m(3) 的元素。这一结果是通过应用多模态融合方法获得的，该方法将同步辐射显微镜技术与离线原子力显微镜相结合。所提出的方法使我们能够找到除质量分数之外的元素浓度，并提供对细胞组成更深入、更完整的了解。我们对对阿霉素敏感 (LoVo-S) 和耐药 (LoVo-R) 的 LoVo 人类结肠癌细胞进行了测量。 LoVo-S 和 LoVo-R 的比较揭示了 Mg 浓度图中的不同模式，LoVo-R 细胞核内和 LoVo-S 细胞核周区域具有较高值。对于其他元素来说，这一特征并不那么明显，这表明镁的区室化可能是耐药细胞的一个重要特征。
We report a method that allows a complete quantitative characterization of whole single cells, assessing the total amount of carbon, nitrogen, oxygen, sodium, and magnesium and providing submicrometer maps of element molar concentration, cell density, mass, and volume. This approach allows quantifying elements down to 10(6) atoms/mu m(3). This result was obtained by applying a multimodal fusion approach that combines synchrotron radiation microscopy techniques with off-line atomic force microscopy. The method proposed permits us to find the element concentration in addition to the mass fraction and provides a deeper and more complete knowledge of cell composition. We performed measurements on LoVo human colon cancer cells sensitive (LoVo-S) and resistant (LoVo-R) to doxorubicin. The comparison of LoVo-S and LoVo-R revealed different patterns in the maps of Mg concentration with higher values within the nucleus in LoVo-R and in the perinuclear region in LoVo-S cells. This feature was not so evident for the other elements, suggesting that Mg compartmentalization could be a significant trait of the drug-resistant cells.