RNA polymerase II subunit D is essential for zebrafish development
RNA polymerase II subunit D is essential for zebrafish development
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DOI：
10.1038/s41598-020-70110-1
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发表时间：
2020-08
影响因子：
4.6
通讯作者：
M. Maeta;M. Kataoka;Yusuke Nishiya;Kazutoyo Ogino;Makoto Kashima;Hiromi Hirata
中科院分区：
文献类型：
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作者：
M. Maeta;M. Kataoka;Yusuke Nishiya;Kazutoyo Ogino;Makoto Kashima;Hiromi Hirata
DNA指导的RNA聚合酶II（polII）由10个核心亚基和2个可分离的亚基组成。该亚复合物是由RPB 4/polr 2d和RPB 7/polr 2g基因分别编码的RPB 4/Polr 2d和RPB 7/Polr 2g的异源二聚体。Rpb 4/Polr 2d在酵母中的功能研究表明，Rpb 4主要在pol II介导的RNA合成中起作用，部分在各种mRNA调节中起作用，包括前mRNA剪接、mRNA的核输出和mRNA的衰变。尽管Rpb 4在酵母和人类之间进化上高度保守，但它在芽殖酵母中的存活是必需的。酿酒酵母，而它是必不可少的生存裂变酵母。粟酒酵母菌黏菌和果蝇为了阐明Rpb 4/Polr 2d是否是脊椎动物发育和生存所必需的，我们产生了polr 2d缺陷的斑马鱼。polr 2d突变体胚胎在受精后11小时（hpf）开始出现体节发生进行性延迟。然后，突变胚胎在15 hpf时显示细胞死亡增加，在48 hpf时显示发育不全，如小眼睛和心脏水肿，并在60 hpf时过早死亡。根据这些发育缺陷，我们的RT-qPCR揭示了管家基因和合子基因的表达在突变体中减少。综上所述，我们得出结论，Rpb 4/Polr 2d是脊椎动物发育所不可或缺的。
DNA-directed RNA polymerase II (pol II) is composed of ten core and two dissociable subunits. The dissociable subcomplex is a heterodimer of Rpb4/Polr2d and Rpb7/Polr2g, which are encoded byRPB4/polr2dandRPB7/polr2ggenes, respectively. Functional studies of Rpb4/Polr2d in yeast have revealed that Rpb4 plays a role primarily in pol II-mediated RNA synthesis and partly in various mRNA regulations including pre-mRNA splicing, nuclear export of mRNAs and decay of mRNAs. Although Rpb4 is evolutionally highly conserved from yeast to human, it is dispensable for survival in budding yeastS. cerevisiae, whereas it was indispensable for survival in fission yeastS. pombe, slime molds and fruit fly. To elucidate whether Rpb4/Polr2d is necessary for development and survival of vertebrate animals, we generatedpolr2d-deficient zebrafish. Thepolr2dmutant embryos exhibited progressive delay of somitogenesis at the onset of 11 h postfertilization (hpf). Mutant embryos then showed increased cell death at 15 hpf, displayed hypoplasia such as small eye and cardiac edema by 48 hpf and prematurely died by 60 hpf. In accordance with these developmental defects, our RT-qPCR revealed that expression of housekeeping and zygotic genes was diminished in mutants. Collectively, we conclude that Rpb4/Polr2d is indispensable for vertebrate development.