An Integrated Strategy for Identifying Targets of Ubiquitin-Mediated Degradation in CD4+ T Cells.
An Integrated Strategy for Identifying Targets of Ubiquitin-Mediated Degradation in CD4+ T Cells.
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DOI：
10.1007/978-1-0716-0266-9_19
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Oliver PM
中科院分区：
文献类型：
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作者：
Field NS;O'Leary CE;Dybas JM;Ding H;Oliver PM
Ubiquitination is a crucial component of many immune processes. While ubiquitin-mediated degradation is essential to T cell activation via T cell receptor signaling, the specific E3 ligases and substrates involved are not well-understood. Here, we describe a strategy integrating RNA, protein, and posttranslational modification datasets to identify targets of ubiquitin-mediated degradation. When integrated, these assays can provide broad insight into how this posttranslational modification regulates protein function and influences T cell biology.
