Role of scalloped in the post-embryonic development of the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae)
Role of scalloped in the post-embryonic development of the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae)
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DOI：
10.1007/s13355-014-0297-4
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发表时间：
2015-02
影响因子：
1.3
通讯作者：
C. Minakuchi;Takahiro Ohde;K. Miura;Toshiharu Tanaka;T. Niimi
中科院分区：
文献类型：
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作者：
C. Minakuchi;Takahiro Ohde;K. Miura;Toshiharu Tanaka;T. Niimi
在果蝇（DrosophilamelanogasterMeigen）的翅发育过程中，扇贝形（sd）与退化（vg）共同起着关键作用。我们以前表明，以及其保守的作用，在翅膀形成的28点瓢虫Henosepilachnavigintioctopunctata（Fabricius），sd具有一个新的功能，蛹蜕皮。然而，负责的分子机制仍然未知。为了确定sd在蛹蜕皮中的功能是否在鞘翅目昆虫中是保守的，我们对sd在赤拟谷盗Tribolium castaneum（Herbst）中的表达和功能进行了分析。castaneum sd（Tc-sd）mRNA从胚胎期到成体期一直持续表达。RNAi介导的Tc-sdin在幼虫阶段的敲低导致在蜕皮间期或幼虫到幼虫蜕皮期间的停滞。在蛹早期注射Tc-sddsRNA，可使蛹期幼虫被捕获为翅短的蛹，而在蛹早期注射Tc-sddsRNA，可使蛹期幼虫被捕获为成虫。这些结果表明，Tc-sd可能参与生长，蜕皮和翅膀的形成。Tc-sd基因敲除对蜕皮激素合成、蜕皮激素级联反应和几丁质合成相关基因的转录水平无明显影响，表明Tc-sd基因敲除对蜕皮激素合成及其级联反应无直接影响。
scalloped(sd) plays a key role in concert withvestigial(vg) in wing development inDrosophila melanogasterMeigen. We previously showed that as well as its conserved role in wing formation in the 28-spotted ladybird beetleHenosepilachnavigintioctopunctata(Fabricius),sdhas a novel function in pupal ecdysis. However, the molecular mechanism responsible remains unknown. To determine whether the function ofsdin pupal ecdysis was conserved among Coleoptera, we performed expression and functional analyses ofsdin the red flour beetle,Tribolium castaneum(Herbst).T. castaneum sd(Tc-sd) mRNA was present continuously from the embryonic stage to the adult. The RNAi-mediated knockdown ofTc-sdin the larval stage resulted in arrest either in the intermolt phase or during larva-to-larva molting. Prepupae injected withTc-sddsRNA were arrested as pupae with shorter wings, while its injection in the early pupal stage resulted in arrest as pharate adults. These results suggested thatTc-sdmay be involved in growth, molting, and wing formation. The transcript levels of the genes involved in ecdysone biosynthesis, the ecdysone cascade, and chitin biosynthesis were not markedly affected by the knockdown ofTc-sd, which indicated thatTc-sdknockdown did not affect ecdysone biosynthesis or its cascade directly.