Mutagenicity and co-mutagenicity of static magnetic fields detected by bacterial mutation assay
Mutagenicity and co-mutagenicity of static magnetic fields detected by bacterial mutation assay
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DOI：
10.1016/s0027-5107(99)00087-1
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发表时间：
1999-06-30
影响因子：
2.3
通讯作者：
Nakagawa, M
中科院分区：
文献类型：
--
作者：
Ikehata, M;Koana, T;Nakagawa, M
用细菌致突变性试验评价强静磁场可能的致突变和共致突变作用。对4株鼠伤寒沙门氏菌(TA98、TA100、TA1535和TA1537)和大肠杆菌WP2 uvrA进行预培养和平板掺入试验，均未检测到5T(T：1T=10000 G)以下的静磁场的诱变潜力。在联合致突变试验中，用不同的化学诱变剂处理大肠杆菌WP2 uvrA细胞，同时暴露在2T或5T的恒磁场中。经N-乙基-N‘-硝基-N-亚硝基-N-亚硝基(ENNG)、N-甲基-N’-硝基-N-亚硝基(MNNG)、甲基甲烷磺酸乙酯(EMS)、4-硝基-N-氧化物(4-NQO)、2-氨基-3-甲基-3-咪唑并[4，5-f]喹酚(IQ)或2-(2-呋喃)-3-(5-硝基-2-呋喃)丙烯酰胺(AF-2)、2-氨基-3-甲基-3-咪唑并[4，5-f]喹酚(IQ)或2-(2-呋喃)-3-(5-硝基-2-呋喃)丙烯酰胺(AF-2)处理后，暴露组细胞突变率明显高于非暴露组。9-氨基吖啶(9-AA)、N-4-氨基胞苷(N-4-氨基胞苷)和2-乙酰氨基荧烯(2-AAF)不受磁场暴露的影响。讨论了磁场共致突变的可能机制。(C)1999 Elsevier Science B.V.保留所有权利。
Possible mutagenic and co-mutagenic effects of strong static magnetic fields were estimated using bacterial mutagenicity test. Mutagenic potential of static magnetic fields up to 5T (T:1T = 10,000 G) was not detected by the bacterial mutagenicity test using four strains of Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) and Escherichia coli WP2 uvrA either in the pre-incubation method or in the plate incorporation method. In the co-mutagenicity test, E. coli WP2 uvrA cells were treated with various chemical mutagens and were simultaneously exposed to a 2T or a 5T static magnetic field. Mutation rate in the exposed group was significantly higher than that in the non-exposed group when cells were treated with N-ethyI-N'-nitro-N-nitrosoguanidine (ENNG), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), ethylmethanesulfonate (EMS), 4-nitroquinoline-N-oxide (4-NQO), 2-amino-3-methyl-3H-imidazo[4,5-f]quinol (IQ) or 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2), The mutagenicity of 2-aminoanthracene (2-AA), 9-aminoacridine (9-AA), N-4-aminocytidine and 2-acetoamidofluorene (2-AAF) was not affected by the magnetic field exposure. Possible mechanisms of the co-mutagenicity of magnetic fields are discussed. (C) 1999 Elsevier Science B.V. All rights reserved.