In situ identification of T6‐positive cells in normal human dermis by immunoelectron microscopy *
In situ identification of T6‐positive cells in normal human dermis by immunoelectron microscopy *
复制标题
通过免疫电子显微镜原位鉴定正常人真皮中的 T6 阳性细胞*
DOI：
10.1111/j.1365-2133.1983.tb04594.x
复制
发表时间：
1983
影响因子：
10.3
通讯作者：
M. C. Mihm
中科院分区：
文献类型：
--
作者：
George F. Murphy;George F. Murphy;A. Bhan;A. Bhan;Terence J. Harrist;Terence J. Harrist;M. C. Mihm;M. C. Mihm
单克隆抗T6抗体与大多数皮质胸腺细胞反应，但不与外周T细胞反应，也与人表皮朗格汉斯细胞反应，如四步免疫过氧化物酶法和免疫电镜所示。为了确定T6阳性细胞是否也存在于正常人真皮中，我们使用这些技术来证明正常人真皮中两种免疫学上不同的组织细胞样细胞群。第一个群体含有缺乏吞噬溶酶体或Birbeck颗粒的细胞。这些细胞与抗T6抗体反应，但不与定义外周T细胞的单克隆抗T3抗体反应，并且主要在真皮淋巴管中及其周围发现。第二种由不与抗T6抗体或抗T3抗体反应的含吞噬溶酶体的细胞组成。
Monoclonal anti‐T6 antibody, which reacts with the majority of cortical thymocytes but not peripheral T cells, also reacts with human epidermal Langerhans cells, as shown by a four‐step immunoperoxidase method and immunoelectron microscopy. To define whether T6‐positive cells are also present in normal human dermis, we used these techniques to demonstrate two immunologically distinct populations of histiocyte‐like cells in normal human dermis. The first population contains cells devoid of phagolysosomes or Birbeck granules. These cells react with anti‐T6 antibody, but not with monoclonal anti‐T3 antibody which defines peripheral T cells, and are found predominantly in and around dermal lymphatic vessels. The second is composed of phagolysosome‐containing cells which do not react with anti‐T6 antibody or anti‐T3 antibody.
DOI：
--
发表时间：
1981
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Murphy,GF;Bhan,AK;Sato,S;Harrist,TJ;MihmJr,MC
通讯作者：
MihmJr,MC
DOI：
10.1073/pnas.78.4.2541
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FITHIAN, E;KUNG, P;EDELSON, R
通讯作者：
EDELSON, R