ROLE OF TEMPERATURE AND ANTICOAGULANT ON IN VITRO SURVIVAL OF BACTERIA IN BLOOD
ROLE OF TEMPERATURE AND ANTICOAGULANT ON IN VITRO SURVIVAL OF BACTERIA IN BLOOD
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DOI：
10.1093/infdis/116.2.238
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发表时间：
1966-01-01
影响因子：
6.4
通讯作者：
STOESSEL, CJ
中科院分区：
文献类型：
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作者：
ELLNER, PD;STOESSEL, CJ
血液中常见的细菌在细菌状态下在室温下比37℃下在柠檬酸血液中存活得更好，在5℃时仍然更好。使用液体(聚苯乙醇磺酸钠)作为抗凝剂可以显著提高某些物种的存活率。除奈瑟氏菌外，所有受试物种在液态血中均能在室温下存活6小时，并可额外存活18小时。在5℃时，讨论了这种抗凝剂在常规血液培养中的使用，特别提到了减少受污染标本的比例。
Bacteria that are commonly encountered in the blood in bacteremic states survived better in citrated blood at room temperature than at 37 C, and still better at 5 C. Markedly improved survival of some species was achieved by using Liquoid (sodium polyanethol sulfonate) as the anticoagulant. All species tested survived 6 hrs at room temperature in Liquoid-blood and, with the exception of Neisseria, 18 additional hr. at 5 C. The use of this anticoagulant in routine blood cultures is discussed with particular reference to reducing the proportion of contaminated specimens.