Role of brain IL-1β on fatigue after exercise-induced muscle damage
Role of brain IL-1β on fatigue after exercise-induced muscle damage
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DOI：
10.1152/ajpregu.00141.2006
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发表时间：
2006-11-01
影响因子：
2.8
通讯作者：
Ghaffar, Abdul
中科院分区：
文献类型：
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作者：
Carmichael, Martin D.;Davis, J. Mark;Ghaffar, Abdul
由各种炎症刺激诱导的脑细胞因子与包括疲劳在内的疾病行为有关。然而，脑细胞因子与运动后疲劳的关系还不清楚。与上坡跑相比，肌肉损伤下坡跑后跑步表现的延迟恢复与脑IL-1 β浓度增加有关。然而，目前还没有系统的评估大脑IL-1 β对运动性肌肉损伤后跑步表现的直接影响。本研究通过脑室内注射IL-1受体拮抗剂（ra;下坡跑步者）或IL-1 β（上坡跑步者）来控制脑IL-1 β活性，检测了脑IL-1 β对上坡和下坡跑步（跑步机或轮跑）后跑步表现的特定作用。将雄性C57 BL/6小鼠分配至以下组：上行-盐水、上行-IL-1 β、下行-盐水或下行-IL-1 ra。小鼠最初在电动跑步机上以22 m/min和-14%或+14%等级跑150 min。（轮笼）或22小时（跑步机），上坡小鼠接受脑室内注射IL-1 β（900 μ g在2 μ l盐水中）或盐水（2 μ l），而下坡赛跑者接受IL-1 ra（1.8 μ g在2 μ l盐水中）或盐水（2 μ l）。之后（2小时），测量跑步表现（轮跑活动和跑步机疲劳）。注射IL-1 β显著降低上坡跑者的跑轮活动（P < 0.01），而注射IL-1 ra显著改善下坡跑者的跑轮活动（P < 0.05）。同样，上坡和下坡运动员中IL-1 β降低，IL-1 ra增加疲劳时间（P < 0.01）。这些结果支持了这样的假设，即增加的大脑IL-1 β在肌肉损伤运动后的疲劳中起着重要作用。
Brain cytokines, induced by various inflammatory challenges, have been linked to sickness behaviors, including fatigue. However, the relationship between brain cytokines and fatigue after exercise is not well understood. Delayed recovery of running performance after muscle-damaging downhill running is associated with increased brain IL-1 beta concentration compared with uphill running. However, there has been no systematic evaluation of the direct effect of brain IL-1 beta on running performance after exercise-induced muscle damage. This study examined the specific role of brain IL-1 beta on running performance (either treadmill or wheel running) after uphill and downhill running by manipulating brain IL-1 beta activity via intracerebroventricular injection of either IL-1 receptor antagonist (ra; downhill runners) or IL-1 beta (uphill runners). Male C57BL/6 mice were assigned to the following groups: uphill-saline, uphill-IL-1 beta, downhill-saline, or downhill-IL-1ra. Mice initially ran on a motor-driven treadmill at 22 m/min and -14% or +14% grade for 150 min. After the run, at 8 h (wheel cage) or 22 h (treadmill), uphill mice received intracerebroventricular injections of IL-1 beta (900 pg in 2 mu l saline) or saline (2 mu l), whereas downhill runners received IL-1ra (1.8 mu g in 2 mu l saline) or saline (2 mu l). Later (2 h), running performance was measured (wheel running activity and treadmill run to fatigue). Injection of IL-1 beta significantly decreased wheel running activity in uphill runners (P < 0.01), whereas IL-1ra improved wheel running in downhill runners (P < 0.05). Similarly, IL-1 beta decreased and Il-1ra increased run time to fatigue in the uphill and downhill runners, respectively (P < 0.01). These results support the hypothesis that increased brain IL-1 beta plays an important role in fatigue after muscle-damaging exercise.