Distribution of Paenibacillus larvae spores inside honey bee colonies and its relevance for diagnosis
Distribution of Paenibacillus larvae spores inside honey bee colonies and its relevance for diagnosis
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DOI：
10.1016/j.jip.2008.05.010
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发表时间：
2008-09-01
影响因子：
3.4
通讯作者：
Belloy, L.
中科院分区：
文献类型：
--
作者：
Gillard, M.;Charriere, J. D.;Belloy, L.
影响蜜蜂群体发育的最重要因素之一是传染病，如由形成孢子的革兰氏阳性细菌类芽孢杆菌幼虫引起的美国幼虫病（AFB）。AFB临床症状的菌落检查非常耗时。此外，感染早期阶段的病变细胞可能容易被忽视。本研究我们研究了是否有可能基于对从蜂巢前收集的不同材料的油分析来确定群体的卫生状况。我们分析了237个蜜蜂样品和67个蜂蜜样品来自71个殖民地位于13个养蜂场的临床AFB发生。我们测试了整个蜂巢内的蜜蜂之间的孢子负荷是否存在差异，以及与其在蜂巢内的位置相关的样品材料最适合于基于培养方法的早期AFB诊断。结果表明，基于蜂蜜样本和在蜂巢入口处收集的蜜蜂分析的诊断价值有限，因为分别只有86%和83%的AFB症状菌落样本呈阳性。从巢、蜜室和边缘框架收集的蜜蜂样品的分析允许检测所有显示AFB临床症状的菌落。微生物学分析表明，从没有AFB临床症状的菌落中收集的样品中有四分之一以上为幼虫阳性。基于这些结果，我们建议通过测试从育雏巢、边缘框架或蜜室采集的蜜蜂样本来调查菌落中的幼虫孢子。(C)2008年爱思唯尔公司All rights reserved.
One of the most important factors affecting the development of honey bee colonies is infectious diseases Such as American foulbrood (AFB) Caused by the spore forming Gram-positive bacterium Paenibacillus larvae. Colony inspections for AFB clinical symptoms are time consuming. Moreover, diseased cells in the early stages of the infection may easily be overlooked. In this Study. we investigated whether it is possible to determine the sanitary status of a colony based oil analyses of different materials collected front the hive. We analysed 237 bee samples and 67 honey samples originating from 71 colonies Situated in 13 apiaries with clinical AFB occurrences. We tested whether a difference in spore load among bees inside the whole hive exists and which sample material related to its location inside the hive was the most appropriate for an early AFB diagnosis based oil the culture method. Results indicated that diagnostics based on analysis of honey samples and bees collected at the hive entrance are of limited value as only 86% and 83%, respectively, of samples from AFB-symptomatic colonies were positive. Analysis of bee samples collected from the brood nest, honey chamber, and edge frame allowed the detection of all colonies showing AFB clinical symptoms. Microbiological analysis showed that more than one quarter of samples collected from colonies without AFB clinical symptoms were positive for P. larvae. Based on these results, we recommend investigating colonies by testing bee samples from the brood nest, edge frame or honey chamber for P. larvae spores. (C) 2008 Elsevier Inc. All rights reserved.