Light-Addressable and Degradable Silica Capsules for Delivery of Molecular Cargo to the Cytosol of Cells
Light-Addressable and Degradable Silica Capsules for Delivery of Molecular Cargo to the Cytosol of Cells
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DOI：
10.1021/cm502472g
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发表时间：
2015-03
影响因子：
8.6
通讯作者：
A. Ott;Xian Yu;Raimo Hartmann;J. Rejman;A. Schütz;Markus Ochs;W. Parak;S. Carregal-Romero
中科院分区：
文献类型：
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作者：
A. Ott;Xian Yu;Raimo Hartmann;J. Rejman;A. Schütz;Markus Ochs;W. Parak;S. Carregal-Romero
可降解和光寻址的二氧化硅胶囊已制备的碳酸钙模板颗粒的基础上。这些胶囊可以装载一系列分子，如抗癌药物（阿霉素）、蛋白质（牛血清白蛋白）和核酸（编码绿色荧光蛋白的mRNA）。这些分子的体外降解和释放进行了论证，并定量比较了几种不同的多层胶囊作为其他运载工具的例子。数据表明水解是二氧化硅胶囊细胞内降解的一种机制。光热加热（通过在二氧化硅壳中整合等离子体纳米颗粒）进一步用于诱导远程分子释放。
Degradable and light-addressable silica capsules have been prepared on the basis of CaCO3 template particles. It was possible to load these capsules with an array of molecules such as anticancer drugs (doxorubicin), proteins (bovine serum albumin), and nucleic acids (mRNA encoding green fluorescent protein). In vitro degradation and release of these molecules was demonstrated and quantitatively compared with several kinds of polyelectrolyte multilayer capsules as examples of other delivery vehicles. The data suggests hydrolysis as a mechanism involved in intracellular degradation of silica capsules. Photothermal heating (by integrating plasmonic nanoparticles in the silica shell) was further used to induce remote molecular release.