Quercetin Improves Neurobehavioral Performance Through Restoration of Brain Antioxidant Status and Acetylcholinesterase Activity in Manganese-Treated Rats
Quercetin Improves Neurobehavioral Performance Through Restoration of Brain Antioxidant Status and Acetylcholinesterase Activity in Manganese-Treated Rats
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DOI：
10.1007/s11064-016-2162-z
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发表时间：
2017-04-01
影响因子：
4.4
通讯作者：
Farombi, Ebenezer O.
中科院分区：
文献类型：
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作者：
Adedara, Isaac A.;Ego, Valerie C.;Farombi, Ebenezer O.
本研究通过对亚慢性染锰大鼠(15 mg/kg体重)和10、20 mg/kg体重(10、20 mg/kg体重)联合灌胃染毒大鼠连续45d的生化和行为学特征的观察，探讨了槲皮素的神经保护机制。在一个新环境中的10分钟试验中，使用视频跟踪软件监测了大鼠的运动行为，同时对大鼠的大脑区域，即下丘脑、大脑和小脑进行了生化分析。结果表明，与槲皮素共处理可显著预防锰致小鼠的运动和运动障碍，特别是总行走距离、全身旋转、最大速度、绝对转角的减少以及不动时间和梳理时间的增加。通过轨迹分析和占用图分析，证实了槲皮素联合治疗后锰处理大鼠的神经行为表现的改善。此外，Quercetin还能减轻锰引起的大鼠下丘脑、大脑和小脑抗氧化酶活性下降，乙酰胆碱酯酶活性、过氧化氢生成和脂质过氧化水平升高。综上所述，Quercetin减轻大鼠锰神经毒性的机制可能与恢复脑乙酰胆碱酯酶活性、增强氧化还原状态和抑制脂质过氧化有关。
The present study investigated the neuroprotective mechanism of quercetin by assessing the biochemical and behavioral characteristics in rats sub- chronically treated with manganese alone at 15 mg/ kg body weight or orally co- treated with quercetin at 10 and 20 mg/ kg body weight for 45 consecutive days. Locomotor behavior was monitored using video- tracking software during a 10- min trial in a novel environment whereas the brain regions namely the hypothalamus, cerebrum and cerebellum of the rats were processed for biochemical analyses. Results indicated that co- treatment with quercetin significantly ( p < 0.05) prevented manganese- induced locomotor and motor deficits specifically the decrease in total distance travelled, total body rotation, maximum speed, absolute turn angle as well as the increase in time of immobility and grooming. The improvement in the neurobehavioral performance of manganese- treated rats following quercetin co- treatment was confirmed by track and occupancy plot analyses. Moreover, quercetin assuaged manganese- induced decrease in antioxidant enzymes activities and the increase in acetylcholinesterase activity, hydrogen peroxide generation and lipid peroxidation levels in the hypothalamus, cerebrum and cerebellum of the rats. Taken together, quercetin mechanisms of ameliorating manganese- induced neurotoxicity is associated with restoration of acetylcholinesterase activity, augmentation of redox status and inhibition of lipid peroxidation in brain of rats.