5-HT and α-m-5-HT attenuate excitatory synaptic transmissions onto the lateral amygdala principal neurons via presynaptic 5-HT1B receptors
5-HT and α-m-5-HT attenuate excitatory synaptic transmissions onto the lateral amygdala principal neurons via presynaptic 5-HT1B receptors
复制标题
5-HT 和 α-m-5-HT 通过突触前 5-HT1B 受体减弱外侧杏仁核主要神经元的兴奋性突触传递
DOI：
10.1016/j.bbrc.2022.07.076
复制
发表时间：
2022
影响因子：
3.1
通讯作者：
Kato Nobuo
中科院分区：
文献类型：
--
作者：
Yamamoto Ryo;Ito Tetsufumi;Furuyama Takafumi;Ono Munenori;Kato Nobuo
越来越多的证据表明，杏仁核中的血清素 (5-HT) 系统对情感状态具有显着影响。基底外侧杏仁复合体中 5-HT 系统的失调会导致情感障碍。为了寻找治疗靶点，5-HT 受体的亚型规范至关重要。本研究旨在鉴定负责 5-HT 介导抑制外侧杏仁核 (LA) 主要神经元 (PN) 兴奋性传递的 5-HT 受体亚型。进行全细胞记录以记录急性大鼠脑切片中的兴奋性突触后电流（EPSC）。我们证实 5-HT 和 α-m-5-HT（一种广泛的 5-HT2 受体激动剂）可减弱 LA PN 中的 EPSC。在利坦色林（一种广泛的 5-HT2 受体拮抗剂）存在下，5-HT 和 α-m-5-HT 的抑制程度保持不变。当 NAS-181（一种选择性 5-HT1B 受体拮抗剂）存在时，5-HT 和 α-m-5-HT 对 EPSC 的抑制程度减弱。 CP93129 是一种选择性 5-HT1B 受体激动剂，可减弱 LA PN 中的 EPSC，并且这种作用在 NAS-181 存在时被消除。此外，CP93129 还增加了 EPSC 的配对脉冲比。因此，我们的结果表明，5-HT 和 α-m-5-HT 减弱了通过突触前 5-HT1B 受体向 LA PN 的兴奋性传递。
Accumulating evidence suggests that the serotonergic (5-HT) system in the amygdala has significant effects on affective states. Dysregulation of the 5-HT system in the basolateral amygdaloid complex causes affective disorders. To search for therapeutic targets, subtype specification of 5-HT receptors is crucial. The present study was undertaken to identify the 5-HT receptor subtype responsible for the 5-HT-mediated suppression of excitatory transmission to principal neurons (PNs) in the lateral amygdala (LA). Whole-cell recordings were performed to record excitatory post synaptic currents (EPSCs) in acute rat brain slices. We confirmed that 5-HT and α-m-5-HT, a broad 5-HT2receptor agonist, attenuated EPSCs in LA PNs. The extent of suppressions by 5-HT and α-m-5-HT remained unchanged in the presence of ritanserin, a broad 5-HT2receptor antagonist. In the presence of NAS-181, a selective 5-HT1Breceptor antagonist, the extent of EPSC suppressions by 5-HT and α-m-5-HT was diminished. CP93129, a selective 5-HT1Breceptor agonist, attenuated EPSCs in LA PNs, and this effect was abolished in the presence of NAS-181. Additionally, the paired-pulse ratio of EPSCs was increased by CP93129. Thus, our results indicate that 5-HT and α-m-5-HT attenuate excitatory transmissions to LA PNs via presynaptic 5-HT1Breceptors.