Epidermal growth factor-mediated mitogen-activated protein kinase3/1 pathway is conducive to in vitro maturation of sheep oocytes.
Epidermal growth factor-mediated mitogen-activated protein kinase3/1 pathway is conducive to in vitro maturation of sheep oocytes.
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表皮生长因子介导的丝裂原激活蛋白激酶3/1途径有利于绵羊卵母细胞的体外成熟
DOI：
10.1371/journal.pone.0120418
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Guo Y
中科院分区：
文献类型：
--
作者：
Ni H;Sheng X;Cui X;Gu M;Liu Y;Qi X;Xing S;Guo Y
表皮生长因子（EGF）已被证明可以促进绵羊卵母细胞的体外成熟，并增强胚胎进一步发育的能力。然而，这种分子机制尚未阐明。在本研究中，我们研究了 EGF 介导的丝裂原激活蛋白激酶 3 和 1 (MAPK3/1) 通路对绵羊卵母细胞体外成熟的影响。将MEK（MAPK激酶）特异性抑制剂U0126添加到成熟培养基中，以不同剂量阻断EGF介导的MAPK3/1通路。然后，检查了绵羊卵母细胞的核成熟。此外，通过体外受精和胚胎发育检查了 EGF 介导的 MAPK3/1 对细胞质成熟的影响。 10−4 mol/l U0126 培养 6 h 后的生发囊泡破裂率 (GVBD) (50.4%) 较对照显着降低 (67.2%, p < 0.05), 药物处理培养 22 h 后的第一极化体 (PB1) 挤出率也较对照显着抑制 (28.6% vs. 48.4%, p < 0.05)。 0.05）。然而，10−6 mol/l U0126 对卵母细胞核成熟有轻微影响。与对照组（68%，p < 0.05）相比，10−4 mol/l U0126 组（54%）体外成熟 22 h 后卵母细胞中 α-微管蛋白的正态分布率显着降低。体外受精后，药物处理后的卵裂率（10−6 mol/l U0126 组为 56.8%，10−4 mol/l U0126 组为 42.6%）与对照组相比显着降低（72.3%，p < 0.01）。 10−4 mol/l U0126 组的囊胚率 (17.6%) 也较对照组 (29.9%, p < 0.05) 显着降低。总的来说，这些结果表明EGF介导的MAPK3/1途径有利于绵羊卵母细胞的体外成熟。
Epidermal growth factor (EGF) has been shown to facilitate the in vitro maturation of sheep oocytes, and enhance embryo’s capability for further development. However, such kind of molecular mechanism has not yet been elucidated. In the present study, we investigated the effect of EGF-mediated mitogen-activated protein kinases 3 and 1 (MAPK3/1) pathway on in vitro maturation of sheep oocytes. U0126, a specific inhibitor of MEK (MAPK kinase), was added into the maturation culture medium to block the EGF-mediated MAPK3/1 pathway with different doses. Then, the nuclear maturation of sheep oocytes was examined. Additionally, the effect of EGF-mediated MAPK3/1 on cytoplasmic maturation was examined though in vitro fertilization and embryonic development. The rate of germinal vesicle breakdown (GVBD) after 6 h of culture with 10−4 mol/l of U0126 (50.4%) was significantly decreased compared with control (67.2%, p < 0.05), and the first polation body (PB1) extrusion rate after 22 h of culture in drug treatment was also significantly inhibited compared with control (28.6% vs. 48.4%, p < 0.05). However, 10−6 mol/l U0126 had slight effect on oocyte nuclear maturation. The normal distribution rate of α-tubulin in the oocytes after 22 h of in vitro maturation was significantly decreased in the 10−4 mol/l U0126 group (54%) compared with control (68%, p < 0.05). After in vitro fertilization, the cleavage rate in drug treatments (56.8% in 10−6 mol/l U0126 group and 42.6% in 10−4 mol/l U0126 group) was significantly decreased compared with control (72.3%, p < 0.01). The blastocyst rate in 10−4 mol/l U0126 group (17.6%) was also significantly decreased compared with control (29.9%, p < 0.05). Collectively, these results suggest that EGF-mediated MAPK3/1 pathway is conducive to in vitro maturation of sheep oocytes.
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DOI：
10.1126/science.1171396
发表时间：
2009-05-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fan HY;Liu Z;Shimada M;Sterneck E;Johnson PF;Hedrick SM;Richards JS
通讯作者：
Richards JS
DOI：
10.1073/pnas.93.14.7032
发表时间：
1996-07-09
影响因子：
11.1
作者：
Choi, TS;Fukasawa, K;VandeWoude, GF
通讯作者：
VandeWoude, GF
影响因子：
4.6
作者：
Norris, Rachael P.;Ratzan, William J.;Jaffe, Laurinda A.
通讯作者：
Jaffe, Laurinda A.
影响因子：
3.6
作者：
Fissore, RA;He, CL;VandeWoude, GF
通讯作者：
VandeWoude, GF
影响因子：
5.3
作者：
Hsieh, Minnie;Lee, Daekee;Conti, Marco
通讯作者：
Conti, Marco