Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans
Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans
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Cofilin 与精子质量相关，并影响人类精子的受精能力。
DOI：
10.1111/andr.12239
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发表时间：
2016-11-01
期刊：
影响因子：
4.5
通讯作者：
Ma, Y. X.
中科院分区：
文献类型：
--
作者：
Chen, S. M.;Chen, X. M.;Ma, Y. X.
精子在雌性生殖道内发生一系列生化变化，统称为精子获能。获能后的精子能与卵透明膜结合，发生顶体反应，使精子进入卵内。肌动蛋白的形成在这些过程中起着重要作用。肌动蛋白在精子获能过程中发生聚合，但在顶体反应前聚合物分散。在本研究中，我们把我们的重点肌动蛋白结合蛋白，cofilin。结果表明，正常精子中cofilin蛋白表达的百分率和强度均显著高于异常精子，且cofilin蛋白表达与精子质量相关。此外，处理与抗cofilin抗体增加精子获能的百分比，并抑制孕酮或A23187诱导的顶体反应的剂量依赖性方式。100 ng/mL抗cofilin抗体的存在显著阻断无透明带仓鼠卵的精子穿透。免疫荧光结果显示，cofilin与F-actin共定位于精子中段，而磷酸化cofilin在精子尾部表达，与F-actin不共定位于精子中段。Western blot结果显示，精子获能过程中磷酸化cofilin增加，顶体反应过程中总cofilin和不溶性部分cofilin增加;免疫荧光结果显示，精子获能过程中顶体cofilin增加。总之，我们的研究表明，cofilin在人类精子中的表达与精子质量相关，cofilin和磷酸化cofilin在受精过程中的变化影响精子获能，顶体反应和精子-卵母细胞融合。
Spermatozoa should undergo a series of biochemical modifications in female reproduction tract, which is collectively called sperm capacitation. The capacitated spermatozoa can bind to the egg zona pellucida, resulting in the occurrence of acrosome reaction which enabled spermatozoa penetrate into the egg. The formation of actin plays an important role in these processes. Actin polymerized during sperm capacitation, but the polymers dispersed before acrosome reaction. In this study, we take our focus on actin-binding protein, cofilin. Our results showed that the % and intensity of sperm expressing cofilin in normal sperm were significantly higher than in abnormal sperm, and the sperm expressing cofilin was correlated with sperm quality. Furthermore, treatment with anti-cofilin antibody increased the percentage of sperm capacitation and inhibited progesterone -or A23187-induced acrosome reaction in a dose-dependent manner. The presence of 100 ng/mL anti-cofilin antibodies markedly blocked the sperm penetration of zona-free hamster eggs. Besides, immunofluorescence results revealed that cofilin was colocalized with F-actin in the midpiece of spermatozoa; however, phospho-cofilin was expressed in the tail rather than in the midpiece of spermatozoa, which was not colocalized with F-actin in spermatozoa. Moreover, western blot revealed that phospho-cofilin increased in sperm capacitation, and the total cofilin and cofilin in insoluble fraction increased in acrosome reaction; immunofluorescence results showed that the amount of cofilin in acrosome increased in sperm capacitation. In conclusion, our study revealed that cofilin expression in human sperm is correlated with sperm quality and the alterations of cofilin and phospho-cofilin in fertilization affects sperm capacitation, acrosome reaction, and spermatozoa-oocyte fusion.