Expression of vascular endothelial growth factor receptors in experimental otitis media in the rat
Expression of vascular endothelial growth factor receptors in experimental otitis media in the rat
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DOI：
10.1080/00016480310001501
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发表时间：
2003-06-01
影响因子：
1.4
通讯作者：
Jung, HH
中科院分区：
文献类型：
--
作者：
Chae, SW;Kim, SJ;Jung, HH
目的-血管通透性增加和内皮细胞生长在渗出性中耳炎 (OME) 的发病机制中很重要，并且已知血管内皮生长因子 (VEGF) 在与 OME 相关的血管通透性增加和血管生成中发挥重要作用。 VEGF 的作用由两种不同的高亲和力受体介导：VEGF 受体-1（VEGFR-1；flm 样酪氨酸激酶-1；flt-1）和 VEGFR-2（包含激酶插入结构域的受体；flk-1）——主要位于血管内皮上。本研究的目的是探讨内毒素诱导的OME大鼠模型中三种形式的VEGFR（-1、-2和-3）的表达。材料与方法-诱导后0、1、3、6和12小时以及1、3、7和14天获取中耳粘膜，使用半定量逆转录酶聚合酶链反应（RT-PCR）和Western blot检测VEGFR mRNA和蛋白的表达。结果RT-PCR显示，内毒素滴注后1小时至3天，VEGFR-1和-2 mRNA的表达上调，并在12小时和第1天表达高峰。所有样品中均未检测到VEGFR-3 mRNA的表达。 Western blotting观察到VEGFR-1和-2蛋白的表达模式与VEGFR-1和-2 mRNA相似，并在第1天观察到峰值表达。在正常或滴注生理盐水的中耳粘膜中均未检测到VEGFR-1和-2蛋白的表达。结论-这些结果表明，在大鼠实验性中耳炎过程中，VEGFR-1和-2均上调，并且这些受体可能在OME积液的产生中发挥不同的作用。
Objective-Increased vascular permeability and endothelial cell growth are important in the pathogenesis of otitis media with effusion (OME) and vascular endothelial growth factor (VEGF) is known to play an important role in the increased vascular permeability and angiogenesis associated with OME. The action of VEGF is mediated by two different high-affinity receptors-VEGF receptor-1 (VEGFR-1; flm-like tyrosine kinase-1; flt-1) and VEGFR-2 (kinase insert domain-containing receptor; flk-1)-predominantly located on the vascular endothelium. The purpose of this study was to investigate the expression of three forms of VEGFR (-1, -2 and -3) in an endotoxin-induced rat model of OME.Material and Methods-Middle ear mucosa were obtained at 0, 1, 3, 6 and 12 h and 1, 3, 7 and 14 days after induction, and the expression of VEGFR mRNA and protein was evaluated using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting.Results-RT-PCR revealed that the expression of VEGFR-1 and -2 mRNA was upregulated between 1 h and 3 days after endotoxin instillation, with peak expression occurring at 12 h and on Day 1. No expression of VEGFR-3 mRNA was detected in any of the samples. Expression patterns of VEGFR-1 and -2 protein observed by Western blotting were similar to those of VEGFR-1 and -2 mRNA and peak expression was observed on Day 1. Expression of VEGFR mRNA or protein was not detected in either normal or saline-instilled middle ear mucosa.Conclusion-These results suggest that both VEGFR-1 and -2 are upregulated during experimental otitis media in the rat and these receptors may play different roles in the production of effusion in OME.