PROTEIN ADSORPTION FROM HUMAN PLASMA IS REDUCED ON PHOSPHOLIPID POLYMERS
PROTEIN ADSORPTION FROM HUMAN PLASMA IS REDUCED ON PHOSPHOLIPID POLYMERS
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DOI：
10.1002/jbm.820251107
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发表时间：
1991-11-01
期刊：
影响因子：
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通讯作者：
ANDERSON, JM
中科院分区：
文献类型：
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作者：
ISHIHARA, K;ZIATS, NP;ANDERSON, JM
通过放射免疫测定和免疫金标记技术，研究了磷脂聚合物、聚（2-甲基丙烯酰氧基乙基磷酰胆碱（MPC）-甲基丙烯酸正丁酯（BMA）或玻璃）对人血浆中蛋白质的吸附。本研究的重点是确定这些材料与人血浆接触后表面的蛋白质的组成和分布。在所有材料上，检测了蛋白质吸附，包括白蛋白、IgG、纤维蛋白原、纤连蛋白、哈格曼因子（因子 XII）、因子 VIII/von Willebrand 因子、高分子量激肽原 (HMWK) 和补体蛋白 C5 的蛋白质吸附量随着 MPC 组成的增加而减少，并且似乎以均匀且均匀分布的方式吸附到表面。因此，我们认为 MPC 部分在抑制蛋白质吸附方面发挥着重要作用。磷脂聚合物的接触表面可能会抑制血栓形成。
Protein adsorption from human plasma was investigated on phospholipid polymers, poly (2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA) or glass by radioimmunoassay and immunogold labeling techniques. In the present studies the focus was to determine the composition and distribution of proteins at the surface of these materials after contact with human blood plasma. On all materials, protein adsorption was detected and included identification of albumin, IgG, fibrinogen, fibronectin, Hageman factor (factor XII), factor VIII/von Willebrand factor, high-molecular-weight kininogen (HMWK) and the complement protein C5. The amount of protein adsorbed decreased with an increase in the MPC composition and appeared to adsorb to the surfaces in a uniform and evenly distributed manner. Therefore, we suggest that MPC moieties play an important role in suppression of protein adsorption. From these findings, it is concluded that the reduction of protein adsorption at the blood contacting surface of phospholipid polymers may result in the inhibition of thrombus formation.