Cutting edge: an inducible sialidase regulates the hyaluronic acid binding ability of CD44-bearing human monocytes.
Cutting edge: an inducible sialidase regulates the hyaluronic acid binding ability of CD44-bearing human monocytes.
复制标题
DOI：
10.4049/jimmunol.162.9.5058
复制
发表时间：
1999-05
影响因子：
4.4
通讯作者：
S. Katoh;T. Miyagi;H. Taniguchi;Y. Matsubara;J. Kadota;A. Tominaga;P. Kincade;S. Matsukura;S. K
中科院分区：
文献类型：
--
作者：
S. Katoh;T. Miyagi;H. Taniguchi;Y. Matsubara;J. Kadota;A. Tominaga;P. Kincade;S. Matsukura;S. K
以前的研究证实，不同程度和类型的糖基化可以解释CD44作为透明质酸受体功能的差异。我们现在已经使用神经氨酸酶治疗得出结论，唾液酸化负面调节人类单核细胞系THP-1和外周血单核细胞上的CD44。这两种类型的细胞在与内毒素共同培养一夜后均表现出较高的受体活性。值得注意的是，唾液酸酶抑制剂2-脱氧-2，3-脱氢-N-乙酰神经氨酸完全阻断了脂多糖诱导的THP-1细胞对透明质酸的识别。此外，这一特征的获得与一种唾液酸酶活性的诱导是平行的。单核细胞可能能够酶促重塑细胞表面CD44，改变其与细胞外基质相互作用的能力。
Previous studies established that variable degrees and types of glycosylation can account for differences in the ability of CD44 to function as a receptor for hyaluronic acid. We have now used neuraminidase treatment to conclude that sialylation negatively regulates CD44 on the human monocytic cell line THP-1 and peripheral blood monocytes. Both of these cell types displayed increased receptor activity after overnight culture with LPS. Of particular interest, the sialidase inhibitor 2-deoxy-2, 3-dehydro-N-acetylneuraminic acid completely blocked the LPS induced recognition of hyaluronic acid by THP-1 cells. Furthermore, acquisition of this characteristic paralleled induction of one type of sialidase activity. Monocytes may be capable of enzymaticly remodeling cell surface CD44, altering their ability to interact with the extracellular matrix.