The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT.
The fractionation, characterization, and subcellular localization of colony-stimulating activities released by the human monocyte-like cell line, GCT.
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DOI：
10.1182/blood.v56.4.717.bloodjournal564717
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发表时间：
1980-10
期刊：
影响因子：
20.3
通讯作者：
J. Dipersio;J. Brennan;M. Lichtman;C. Abboud;F. Kirkpatrick
中科院分区：
文献类型：
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作者：
J. Dipersio;J. Brennan;M. Lichtman;C. Abboud;F. Kirkpatrick
GCT是一种人类单核细胞样细胞系，已被证明对小鼠和人骨髓释放不同的生化集落刺激活性(CSA)。这些似乎是具有关键二硫键的高碘酸盐敏感蛋白。一种分子质量为145,000道尔顿，可刺激巨噬细胞集落生长，与一种30,000道尔顿的分子有关，该分子也能刺激小鼠的生长。用等电聚焦和梯度聚丙烯酰胺凝胶电泳法可以从3万道尔顿的小鼠CsA中分离出3万道尔顿的人骨髓CsA。这一区别与我们先前发现的与抗人尿液脑脊液抗体的差异中和一致。30,000道尔顿的CSA刺激人骨髓中的中性粒细胞、中性粒细胞单核细胞和嗜酸性粒细胞集落的生长，但在小鼠中只刺激中性粒细胞和中性粒细胞单核细胞的集落生长。GCT细胞的亚细胞分离表明，存在主要与细胞胞浆相关的预先形成的CSA池，其表观分子量与其分泌的对应物相似。
GCT, a human monocyte-like cell line, has been shown to release biochemically distinct colony-stimulating activities (CSAs) for mouse and human marrows. These appear to be periodate-sensitive proteins with critical disulfide bonds. One, of molecular weight 145,000 daltons, stimulates macrophagic colony growth and is related to a 30,000-dalton molecule that also stimulates mouse growth. A 30,000-dalton CSA for human marrow can be separated from the 30,000-dalton mouse CSA by isoelectric focusing and gradient polyacrylamide gel electrophoresis. This distinction agrees with our previous finding of differential neutralization with anti-human urinary CSF antibody. The 30,000-dalton CSAs stimulate neutrophil, neutrophil-monocyte, and eosinophil colony growth in human marrow but only neutrophil and neutrophil-monocyte colonies in the mouse. Subcellular fractionation of GCT cells indicates that there are pools of preformed CSAs primarily associated with the cell cytosol that have similar apparent molecular weights to their secreted counterparts.