Development of new stem cell-based technologies for carnivore reproduction research.
Development of new stem cell-based technologies for carnivore reproduction research.
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开发基于干细胞的新技术用于食肉动物繁殖研究。
DOI：
10.1111/j.1439-0531.2009.01396.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Meyers-Wallen,V
中科院分区：
文献类型：
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作者：
Travis,AJ;Kim,Y;Meyers-Wallen,V
内容基于干细胞的新生殖技术为食肉动物物种提供了几个潜在的好处。例如，猫和狗胚胎干细胞系的发展将允许产生转基因动物模型，这可以用于促进兽医和人类健康。精原干细胞移植(SSCT)和睾丸异种移植等技术为繁殖具有遗传价值的个体男性提供了新的方法，即使他们应该在产生精子之前死亡。因此，这些技术可能会应用于濒危食肉动物物种的保护，以及生物医学研究。最近，我们实验室成功地在狗身上进行了SSCT，其中一只受体犬产生了供体基因来源的精子。异种睾丸移植已被用于从猫和雪貂的青春期前睾丸组织中产生精子。这些早期步骤不仅加强了对干细胞技术的研究，而且还需要对食肉动物辅助生殖的补充技术进行更多的研究，以便实现最广泛的研究和临床益处。
ContentsNew reproductive technologies based on stem cells offer several potential benefits to carnivore species. For example, development of lines of embryonic stem cells in cats and dogs would allow for the generation of transgenic animal models, which could be used to advance both veterinary and human health. Techniques such as spermatogonial stem cell transplantation (SSCT) and testis xenografting offer new approaches to propagate genetically valuable individual males, even if they should die before producing sperm. These techniques might therefore have application to the conservation of endangered species of carnivores, as well as to biomedical research. Recently, our laboratory has successfully performed SSCT in the dog, with a recipient dog producing sperm of donor genetic origin. Testis xenografting has been used to produce sperm from pre‐pubertal testis tissue from both cats and ferrets. These early steps reinforce the need not only for research on stem cell technologies, but also for additional research into complementary technologies of assisted reproduction in carnivores, so that the widest array of research and clinical benefits can be realized.