Physicochemical characterization of alkali pretreated sugarcane tops and optimization of enzymatic saccharification using response surface methodology
Physicochemical characterization of alkali pretreated sugarcane tops and optimization of enzymatic saccharification using response surface methodology
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DOI：
10.1016/j.renene.2013.07.041
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发表时间：
2014-02-01
期刊：
影响因子：
8.7
通讯作者：
Pandey, Ashok
中科院分区：
文献类型：
--
作者：
Sindhu, Raveendran;Kuttiraja, Mathiyazhakan;Pandey, Ashok
在实验室高压釜中，用 3% NaOH 在 121 摄氏度下对甘蔗顶部进行碱预处理 60 分钟。根据 Box-Behnken 设计，使用响应面法研究了固体负载、酶负载、孵育时间和表面活性剂浓度对酶促糖化的影响。在优化条件下，从预处理的生物质中回收了 77.5% 的糖。该产量比未经处理的甘蔗顶高出七倍。通过这种预处理方法去除了大量的木质素（90%）。通过 XRD、FTIR 和 SEM 对天然甘蔗顶和碱预处理甘蔗顶进行了物理化学表征，并监测了化学成分的变化。 X射线衍射图显示碱预处理生物质的结晶度高于天然生物质。 (C) 2013 Elsevier Ltd. 保留所有权利。
Alkali pretreatment of sugarcane tops was carried out with 3% NaOH for 60 min at 121 degrees C in a laboratory autoclave. The effect of solid loading, enzyme loading, incubation time and surfactant concentration on enzymatic saccharification was studied using a response surface method according to Box-Behnken design. Under optimized conditions 77.5% sugar was recovered from the pretreated biomass. This yield was seven times higher than that obtained with untreated sugarcane tops. A substantial amount of lignin (90%) was removed by this pretreatment method. Physicochemical characterization of native and alkali pretreated sugarcane tops were carried out by XRD, FTIR and SEM and the changes in the chemical composition were also monitored. The X-ray diffraction profile showed that the degree of crystallinity was higher for alkali pretreated biomass than that for native. (C) 2013 Elsevier Ltd. All rights reserved.