Expression of c-Fos in arcuate nucleus induced by electroacupuncture: Relations to neurons containing opioids and glutamate
Expression of c-Fos in arcuate nucleus induced by electroacupuncture: Relations to neurons containing opioids and glutamate
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DOI：
10.1016/j.brainres.2007.06.042
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发表时间：
2007-08-29
期刊：
影响因子：
2.9
通讯作者：
Longhurst, John C.
中科院分区：
文献类型：
--
作者：
Guo, Zhi-Ling;Longhurst, John C.
电针内关-肩室穴（P5-P6，覆盖正中神经）可能通过阿片系统减弱交感兴奋性反射。下丘脑弓状核（ARC）是产生阿片肽的重要脑区。生理学研究表明，对EA的主要反应是ARC中的兴奋，并且在P5-P6的EA期间，从ARC到腹外侧导水管周围灰质的兴奋性投射有助于抑制交感兴奋性心血管反射。这些结果提示，电针激活的ARC神经元也可能含有兴奋性神经递质。因此，本研究通过检测即时早期基因c-Fos、阿片肽和囊泡谷氨酸转运蛋白3（VGLUT 3），评估了在P5-P6时电针诱导的ARC激活与阿片系统和谷氨酸的关系。为了提高检测含有阿片肽的胞体，秋水仙碱（90-100 μ g/kg）在猫EA或假手术对照前28-30小时给药。与对照组（n=5）相比，电针组ARC区c-Fos阳性细胞和c-Fos与β-内啡肽、脑啡肽和VGLUT 3双标神经元数量均显著增加（n = 6;均P < 0.05）。此外，三重标记的神经元c-Fos，β-内啡肽和VGLUT 3在该区域的EA刺激后，注意到，而不是在对照组。因此，在P5-P6的EA激活ARC中的神经元，其中一些含有阿片类药物以及谷氨酸盐或两者兼而有之。结果表明，在P5-P6电针有可能影响ARC神经元含有多种神经元物质，随后调节心血管功能。(c)2007 Elsevier B. V.保留所有权利。
Electroacupuncture (EA) at the Neiguan-Jianshi acupoints (P5-P6, overlying the median nerve) attenuates sympathoexcitatory reflexes probably through the opioid system. The arcuate nucleus (ARC) within hypothalamus is an important brain area that produces opioid peptides. Physiological studies have demonstrated that the predominant response to EA is excitation in the ARC and that excitatory projections from the ARC to the ventrolateral periaqueductal gray during EA at P5-P6 contribute to inhibition of sympathoexcitatory cardiovascular reflexes. These data imply that ARC neurons activated by EA also may contain excitatory neurotransmitters. Thus, the present study evaluated activation of the ARC induced by EA at P5-P6, in relation to the opioid system and glutamate, by detecting c-Fos, an immediate early gene, opioid peptides and vesicular glutamate transporter 3 (VGLUT3). To enhance detection of perikarya containing the opioid peptides, colchicine (90-100 mu g/kg) was administered in cats 28-30 h before EA or the sham-operated control. EA was performed at P5-P6 for 30 min. Compared to controls (n=5), c-Fos-positive cells and neurons double-labeled with c-Fos and beta-endorphin, enkephalin or VGLUT3 in the ARC were significantly increased in EA-treated cats (n = 6; all P < 0.05). Moreover, neurons triple-labeled with c-Fos, beta-endorphin and VGLUT3 were noted in this region following EA stimulation, but not in controls. Thus, EA at P5-P6 activates neurons in the ARC, some of which contain opioids as well as glutamate or both. The results imply that EA at P5-P6 has the potential to influence ARC neurons containing multiple neuronal substances that subsequently modulate cardiovascular function. (c) 2007 Elsevier B.V. All rights reserved.