Volume electron microscopy.
Volume electron microscopy.
复制标题
DOI：
10.1038/s43586-022-00131-9
复制
发表时间：
2022-07-07
期刊：
影响因子：
--
通讯作者：
Collinson LM
中科院分区：
文献类型：
--
作者：
Peddie CJ;Genoud C;Kreshuk A;Meechan K;Micheva KD;Narayan K;Pape C;Parton RG;Schieber NL;Schwab Y;Titze B;Verkade P;Aubrey A;Collinson LM
生命存在于三维空间中，但直到世纪之交，大多数电子显微镜方法只能提供二维图像数据。最近，出现了能够深入研究细胞和组织结构的电子显微镜技术，统称为体积电子显微镜（vEM）。vEM的发展被称为一场静悄悄的革命，因为该领域是从已建立的透射和扫描电子显微镜技术发展而来的，因此早期的出版物主要集中在生物科学应用上，而不是潜在的技术突破。然而，随着vEM在整个生物科学领域的爆炸式增长以及体积、分辨率、吞吐量和易用性的快速进步，现在是向新受众介绍该领域的时候了。在本书中，我们将介绍不同的vEM成像模式、每种模式所伴随的专用样本处理和图像分析管道以及数据中显示的信息类型。我们展示了vEM在生物科学中的关键应用，这些应用有助于取得突破性发现，并考虑限制和未来方向。我们的目标是向新用户展示vEM如何在他们自己的研究领域支持发现科学，并激发更广泛的技术吸收，最终使其完全采用主流生物成像。
Life exists in three dimensions, but until the turn of the century most electron microscopy methods provided only 2D image data. Recently, electron microscopy techniques capable of delving deep into the structure of cells and tissues have emerged, collectively called volume electron microscopy (vEM). Developments in vEM have been dubbed a quiet revolution as the field evolved from established transmission and scanning electron microscopy techniques, so early publications largely focused on the bioscience applications rather than the underlying technological breakthroughs. However, with an explosion in the uptake of vEM across the biosciences and fast-paced advances in volume, resolution, throughput and ease of use, it is timely to introduce the field to new audiences. In this Primer, we introduce the different vEM imaging modalities, the specialized sample processing and image analysis pipelines that accompany each modality and the types of information revealed in the data. We showcase key applications in the biosciences where vEM has helped make breakthrough discoveries and consider limitations and future directions. We aim to show new users how vEM can support discovery science in their own research fields and inspire broader uptake of the technology, finally allowing its full adoption into mainstream biological imaging.
影响因子：
5.4
作者：
Gabarre S;Vernaillen F;Baatsen P;Vints K;Cawthorne C;Boeynaems S;Michiels E;Vandael D;Gounko NV;Munck S
通讯作者：
Munck S
影响因子：
2
作者：
Guerin, Christopher J.;Lippens, Saskia
通讯作者：
Lippens, Saskia
影响因子：
5
作者：
Lane R;Wolters AHG;Giepmans BNG;Hoogenboom JP
通讯作者：
Hoogenboom JP