Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment
Whole genome analysis of epizootic hemorrhagic disease virus identified limited genome constellations and preferential reassortment
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DOI：
10.1099/vir.0.059659-0
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发表时间：
2014-02-01
影响因子：
3.8
通讯作者：
Hause, Ben M.
中科院分区：
文献类型：
--
作者：
Anbalagan, Srivishnupriya;Cooper, Elyse;Hause, Ben M.
动物流行性出血症病毒（EHDV）是一种由库蠓传播的环状病毒，可引起野生和家养反刍动物的出血症。对2008年至2012年分离的44株EHDV进行了完全测序和遗传学分析。除2012年血清型6型病毒占63%外，血清型2型病毒均为优势血清型。血清1型和2型病毒VP1、VP3、VP4、VP6、NS1、NS2和NS3区段之间的高遗传相似性（>94%同一性）阻止了这些区段的重配事件的鉴定。此外，在VP2、VP5和VP7的血清型内几乎没有遗传多样性（>96%同一性）。1型和2型病毒的VP2、VP5和VP7片段在同源血清型内观察到优先重配。相比之下，6型病毒都是重配株，含有源自外来6型的VP 2和VP 5，其余片段与2型病毒最相似。这些结果表明，1型和2型病毒之间的重配需要保守的VP2，VP5和VP7段星座，而6型病毒只需要VP2和VP5，并限于2型谱系VP7。由于6型VP2和VP5片段仅在具有2型衍生的VP7的病毒中鉴定，这些结果表明2型和6型VP7蛋白之间的功能互补。
Epizootic hemorrhagic disease virus (EHDV) is a Culicoides transmitted orbivirus that causes haemorrhagic disease in wild and domestic ruminants. A collection of 44 EHDV isolated from 2008 to 2012 was fully sequenced and analysed phylogenetically. Serotype 2 viruses were the dominant serotype all years except 201 2 when serotype 6 viruses represented 63% of the isolates. High genetic similarity (>94% identity) between serotype 1 and 2 virus VP1, VP3, VP4, VP6, NS1, NS2 and NS3 segments prevented identification of reassortment events for these segments. Additionally, there was little genetic diversity (>96% identity) within serotypes for VP2, VP5 and VP7. Preferential reassortment within the homologous serotype was observed for VP2, VP5 and VP7 segments for type 1 and type 2 viruses. In contrast, type 6 viruses were all reassortants containing VP2 and VP5 derived from an exotic type 6 with the remaining segments most similar to type 2 viruses. These results suggest that reassortment between type 1 and type 2 viruses requires conservation of the VP2, VP5 and VP7 segment constellation while type 6 viruses only require VP2 and VP5 and are restricted to type 2-lineage VP7. As type 6 VP2 and VP5 segments were exclusively identified in viruses with type 2-derived VP7, these results suggest functional complementation between type 2 and type 6 VP7 proteins.