ANDROGEN RECEPTOR EXPRESSION IN DEVELOPING MALE REPRODUCTIVE-ORGANS
ANDROGEN RECEPTOR EXPRESSION IN DEVELOPING MALE REPRODUCTIVE-ORGANS
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DOI：
10.1210/endo-128-6-2867
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发表时间：
1991-06-01
期刊：
影响因子：
4.8
通讯作者：
CUNHA, GR
中科院分区：
文献类型：
--
作者：
COOKE, PS;YOUNG, P;CUNHA, GR
通过H - 3 - 双氢睾酮类固醇放射自显影技术，确定了雄激素受体（AR）在发育中的雄性BALB/c小鼠生殖器官中的分布情况。对从胎龄13天（妊娠期 = 19 - 20天）到出生后10天的小鼠的输出小管、泌尿生殖窦（UGS）和中肾管及其衍生物附睾、输精管、精囊、凝固腺、前列腺和尿道球腺进行了检测。在所有检测时间点，所有器官的间充质/基质细胞中均含有AR。在妊娠期13 - 14天或16天，中肾管和泌尿生殖窦的上皮细胞中不含AR。输出小管是发育过程中雄性生殖道上皮细胞中AR表达的首个部位；该器官在第16天及之后的所有时间点上皮细胞中均有AR。附睾和输精管从妊娠期第19天开始上皮细胞中含有AR。精囊和凝固腺上皮在出生时无AR，在第1天变为弱AR +，在第2天及之后为强AR +。前列腺上皮在第4天之前无AR，第4天可观察到一些阳性上皮细胞；前列腺上皮在第6天及之后为强AR +。最后一个开始表达上皮AR的器官是尿道球腺；该上皮细胞直到出生后第8天才明显呈现AR +。总之，这些结果表明，雄性生殖道中上皮AR的初始表达呈现明显的时间顺序，并按照从头端到尾端的方向进行。上皮AR首先出现在输出小管，随后在中肾管衍生的器官中开始初始上皮AR表达，最后在泌尿生殖窦衍生的器官中表达。控制初始上皮AR表达的因素尚不清楚，但间充质可能参与其中。
The distribution of androgen receptors (AR) in developing male BALB/c mouse reproductive organs was determined by H-3-dihydrotestosterone steroid autoradiography. The efferent ductules, urogenital sinus (UGS) and Wolffian ducts, and their derivatives, the epididymis, ductus deferens, seminal vesicles, coagulating glands, prostate and bulbouretheral glands, were examined in mice from 13-days fetal (gestation = 19-20 days) to 10 days postnatal. All organs contained AR in their mesenchymal/stromal cells at all times examined. The Wolffian ducts and UGS did not contain epithelial AR on days 13-14 or 16 of gestation. The efferent ductule was the first site of epithelial AR expression in the male tract during development; this organ had epithelial AR on day 16 and at all subsequent times. The epididymis and ductus deferens contained epithelial AR beginning on day 19 of gestation. Seminal vesicle and coagulating gland epithelium was AR- at birth, became weakly AR+ on day 1, and was strongly AR+ on day 2 and subsequently. Prostatic epithelium was AR- up to day 4, when some positive epithelial cells were seen; the prostatic epithelium was strongly AR+ on day 6 and subsequently. The last organ to begin expressing epithelial AR was the bulbouretheral gland; this epithelium did not become clearly AR+ until day 8 postnatally. In summary, these results indicate that initial epithelial AR expression in the male reproductive tract occurs in a clear temporal sequence and proceeds in a cranial-caudal direction. Epithelial AR first appear in the efferent ductules, followed by initial epithelial AR expression in Wolffian-derived organs and finally in the UGS-derived organs. The factors controlling initial epithelial AR expression are unclear, but mesenchyme may be involved.