BASIC FIBROBLAST GROWTH-FACTOR (BFGF) GENE-EXPRESSION AND PROTEIN-PRODUCTION DURING PUBERTAL DEVELOPMENT OF THE SEMINIFEROUS TUBULE - FOLLICLE-STIMULATING HORMONE-INDUCED SERTOLI-CELL BFGF EXPRESSION
BASIC FIBROBLAST GROWTH-FACTOR (BFGF) GENE-EXPRESSION AND PROTEIN-PRODUCTION DURING PUBERTAL DEVELOPMENT OF THE SEMINIFEROUS TUBULE - FOLLICLE-STIMULATING HORMONE-INDUCED SERTOLI-CELL BFGF EXPRESSION
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DOI：
10.1210/en.131.6.2928
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发表时间：
1992-12-01
期刊：
影响因子：
4.8
通讯作者：
SKINNER, MK
中科院分区：
文献类型：
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作者：
MULLANEY, BP;SKINNER, MK
研究了碱性成纤维细胞生长因子（bFGF）在睾丸生长发育过程中作为细胞间相互作用的中介的潜在作用。采用核酸酶保护分析评价bFGF基因在睾丸及其他男性生殖道组织中的表达。bFGF在20日龄大鼠的精囊、前列腺、附睾以及睾丸中表达明显，表达水平较低。测定bFGF在全睾丸和分离体细胞类型中的发育表达。从青春期前、青春期中期和青春期晚期的大鼠睾丸中分离出间充质来源的小管周围细胞和上皮样支持细胞。在整个睾丸中，bFGF的表达在青春期前睾丸发育早期占优势，并随着性成熟而降低。新分离的小管周围细胞和支持细胞在青春期发育期间表达bFGF的水平相对恒定，在青春期后期略有抑制。新鲜分离的成熟间质细胞也表达低水平的bFGF。培养的支持细胞和小管周围细胞产生bfgf样蛋白，包括18千道尔顿和24千道尔顿形式。有趣的是，FSH增加了支持细胞bFGF基因的表达和蛋白质的产生。此前，FSH和bFGF已被证明能刺激未成熟的支持细胞生长。目前的研究结果表明，卵泡刺激素调节睾丸和支持细胞增殖的能力可能部分是通过局部bFGF的产生和作用间接介导的。bFGF以前也被证明存在于发育中的生发细胞中。因此，fsh诱导的支持细胞bFGF表达可能介导支持细胞与生发细胞的相互作用，参与控制生精过程。观察表明bFGF的存在与精小管体细胞群的活跃生长一致。bFGF在精小管中的潜在作用包括小管的血管生成、青春期前的支持细胞增殖和介导支持细胞与生发细胞的相互作用。
The potential role of basic fibroblast growth factor (bFGF) as a mediator of cell-cell interactions in the growth and development of the testis was examined. Nuclease protection analysis was used to evaluate bFGF gene expression in the testis and other male reproductive tract tissues. bFGF expression was evident in seminal vesicle, prostate, epididymis, and, at low levels, testis of 20-day-old rats. The developmental expression of bFGF in whole testis and isolated somatic cells types was determined. Mesenchymal-derived peritubular cells and epithelial-like Sertoli cells were isolated from prepubertal, midpubertal, and late pubertal rat testes. In whole testis, bFGF expression is predominant early in prepubertal testicular development and decreases with sexual maturity. Both freshly isolated peritubular and Sertoli cells express bFGF at relatively constant levels during pubertal development, with a slight suppression at the late pubertal stages. Freshly isolated mature Leydig cells also expressed low levels of bFGF. Cultured Sertoli and peritubular cells produced bFGF-like proteins, including 18- and 24-kilodalton forms. Interestingly, FSH increased Sertoli cell bFGF gene expression and protein production. Previously, FSH and bFGF have been shown to stimulate immature Sertoli cell growth. The results of the current study suggest that the ability of FSH to regulate testis and Sertoli cell proliferation may in part be indirectly mediated through the local production and action of bFGF. bFGF has also previously been shown to localize in developing germinal cells. Therefore, FSH-induced Sertoli cell bFGF expression may mediate Sertoli-germinal cell interactions involved in the control of the spermatogenic process. Observations demonstrate the presence of bFGF at a time coinciding with active growth of the somatic cell populations of the seminiferous tubule. Potential roles for bFGF in the seminiferous tubule to consider include angiogenesis of the tubule, prepubertal Sertoli cell proliferation, and mediating Sertoli-germinal cell interactions.