In vivo photoacoustic imaging of major blood vessels in the pancreas and liver during surgery
In vivo photoacoustic imaging of major blood vessels in the pancreas and liver during surgery
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DOI：
10.1117/1.jbo.24.12.121905
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发表时间：
2019-12-01
影响因子：
3.5
通讯作者：
Bell, Muyinatu A. Lediju
中科院分区：
文献类型：
--
作者：
Kempski, Kelley M.;Wiacek, Alycen;Bell, Muyinatu A. Lediju
腹部手术存在相当大的胃肠道和腹腔内出血风险，这可能导致患者死亡。光声成像是克服这一挑战的一种解决方案，它可以在手术过程中提供主要血管的可视化。我们研究了体内血管可视化用于光声引导肝脏和胰腺手术的可行性。对两只猪进行剖腹手术后，成功实现了这两个腹部器官主要血管的体内光声成像。使用机器人控制的超声（US）探头和彩色多普勒成像的三维光声成像来确认血管位置。使用 20 至 40 mJ 的能量对体内肝脏中的血管进行可视化，从而产生 10 至 15 dB 的血管对比度。同样，36 mJ 的能量足以以高达 17.3 dB 的对比度可视化胰腺中的血管。我们观察到，当光源遇到肝脏中的主要血管时，光声信号更加集中。该观察结果可用于区分图像平面中的主要血管和与周围组织中的较小血管相关的更扩散的信号。对切除的胰腺和肝脏组织进行术后组织病理学分析，以探讨可能的激光相关损伤。当超声探头固定并且光源用于询问手术工作空间时，光声引导腹部手术的结果通常是有希望的。这些发现还适用于可能受益于光声引导肝脏和胰腺介入成像的其他手术（例如，活检和引导肝脏中的射频消融病灶）。 (C) 作者。由 SPIE 根据 Creative Commons Attribution 4.0 Unported 许可证发布。
Abdominal surgeries carry considerable risk of gastrointestinal and intra-abdominal hemorrhage, which could possibly cause patient death. Photoacoustic imaging is one solution to overcome this challenge by providing visualization of major blood vessels during surgery. We investigate the feasibility of in vivo blood vessel visualization for photoacoustic-guided liver and pancreas surgeries. In vivo photoacoustic imaging of major blood vessels in these two abdominal organs was successfully achieved after a laparotomy was performed on two swine. Three-dimensional photoacoustic imaging with a robot-controlled ultrasound (US) probe and color Doppler imaging were used to confirm vessel locations. Blood vessels in the in vivo liver were visualized with energies of 20 to 40 mJ, resulting in 10 to 15 dB vessel contrast. Similarly, an energy of 36 mJ was sufficient to visualize vessels in the pancreas with up to 17.3 dB contrast. We observed that photoacoustic signals were more focused when the light source encountered a major vessel in the liver. This observation can be used to distinguish major blood vessels in the image plane from the more diffuse signals associated with smaller blood vessels in the surrounding tissue. A postsurgery histopathological analysis was performed on resected pancreatic and liver tissues to explore possible laser-related damage. Results are generally promising for photoacoustic-guided abdominal surgery when the US probe is fixed and the light source is used to interrogate the surgical workspace. These findings are additionally applicable to other procedures that may benefit from photoacoustic-guided interventional imaging of the liver and pancreas (e.g., biopsy and guidance of radiofrequency ablation lesions in the liver). (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.