Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene
Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene
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DOI：
10.5511/plantbiotechnology.25.69
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Shibata, Michio
中科院分区：
文献类型：
--
作者：
Aida, Ryutaro;Narumi, Takako;Shibata, Michio
我们研究了烟草醇脱氢酶基因 (NtADH-5'UTR) 的 5'-非翻译区 (UTR) 作为菊花 (Chrysanthemum morifolium) 和蓝猪草 (Torenia fournieri) 翻译增强子的效率。引入三种构建体：对照“ADH-”花椰菜花叶病毒35S RNA (CaMV 35S)启动子::β-葡萄糖醛酸酶(GUS)； NtADH-5'UTR，带有间隔区“ADH+S”，CaMV 35S 启动子::NtADH-5'UTR::25-bp 间隔区::GUS；和无间隔区的 NtADH-5'UTR、“ADH+”、CaMV 35S 启动子::NtADH-5'UTR::GUS。菊花中ADH+S和ADH+的最高GUS活性分别是ADH植物的45倍和190倍，蓝猪耳分别是ADH植物的12和22倍。 NtADH-5'UTR 提高了两个物种的翻译效率。当最低翻译效率设置为1时，菊花的相对翻译效率为1至15（ADH-）、47至568（ADH+S）和267至1360（ADH+），而菊花的相对翻译效率为1至3（ADH-）、47至114（ADH+S）和85至226（ADH+）。托雷尼亚。 NtADH-5'UTR 将促进菊花和蓝猪耳的实际育种和基础遗传学研究。
We investigated the efficiency of the 5'-untranslated region (UTR) of the tobacco alcohol dehydrogenase gene (NtADH-5'UTR) as a translational enhancer in chrysanthemum (Chrysanthemum morifolium) and torenia (Torenia fournieri). Three constructs were introduced: control, "ADH-," cauliflower mosaic virus 35S RNA (CaMV 35S) promoter::beta-glucuronidase (GUS); NtADH-5'UTR with a spacer, "ADH+S," CaMV 35S promoter::NtADH-5'UTR::25-bp spacer::GUS; and NtADH-5'UTR with no spacer, "ADH+," CaMV 35S promoter:: NtADH-5'UTR::GUS. The highest GUS activity in ADH+S and ADH+ for chrysanthemum was about 45 and 190 times, respectively, and for torenia was 12 and 22 times, respectively, than that for the ADH-plants. NtADH-5'UTR enhanced translational efficiency in both species. With the lowest translational efficiency set to 1, the relative translational efficiencies were 1 to 15 (ADH-), 47 to 568 (ADH+S), and 267 to 1360 (ADH+) for chrysanthemum, and 1 to 3 (ADH-), 47 to 114 (ADH+S), and 85 to 226 (ADH+) for torenia. NtADH-5'UTR would facilitate practical breeding and fundamental genetic research in chrysanthemum and torenia.