NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus
NADPH oxidases regulate septin-mediated cytoskeletal remodeling during plant infection by the rice blast fungus
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DOI：
10.1073/pnas.1217470110
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发表时间：
2013-02-19
影响因子：
11.1
通讯作者：
Talbot, Nicholas J.
中科院分区：
文献类型：
--
作者：
Ryder, Lauren S.;Dagdas, Yasin F.;Talbot, Nicholas J.
稻瘟病菌通过一种称为附着胞的特殊细胞感染植物，附着胞利用膨压力驱动一个刚性的穿透钉穿过水稻叶片角质层。在这里，我们表明，NADPH氧化酶（Nox）是必要的septin介导的重新定位的F-肌动蛋白细胞骨架，以促进角质层破裂和植物细胞入侵。我们报告说，Nox 2-NoxR复杂的空间组织异聚隔蛋白环在附着胞孔，所需的装配的环形F-肌动蛋白网络的点的渗透钉出现。植物感染期间皮质F-肌动蛋白网络的维持独立地需要Nox 1，这是第二种NADPH氧化酶，它是穿透菌丝伸长所必需的。附着胞中F-肌动蛋白的组织被破坏的抗氧化剂的应用，而latrunculin介导的解聚附着胞F-肌动蛋白的活性氧竞争性抑制，提供证据表明，真菌NADPH氧化酶直接控制septin和F-肌动蛋白的动力学调节活性氧的合成。
The rice blast fungus Magnaporthe oryzae infects plants with a specialized cell called an appressorium, which uses turgor to drive a rigid penetration peg through the rice leaf cuticle. Here, we show that NADPH oxidases (Nox) are necessary for septin-mediated reorientation of the F-actin cytoskeleton to facilitate cuticle rupture and plant cell invasion. We report that the Nox2-NoxR complex spatially organizes a heteroligomeric septin ring at the appressorium pore, required for assembly of a toroidal F-actin network at the point of penetration peg emergence. Maintenance of the cortical F-actin network during plant infection independently requires Nox1, a second NADPH oxidase, which is necessary for penetration hypha elongation. Organization of F-actin in appressoria is disrupted by application of antioxidants, whereas latrunculin-mediated depolymerization of appressorial F-actin is competitively inhibited by reactive oxygen species, providing evidence that regulated synthesis of reactive oxygen species by fungal NADPH oxidases directly controls septin and F-actin dynamics.