Toxicity and imaging of multi-walled carbon nanotubes in human macrophage cells
Toxicity and imaging of multi-walled carbon nanotubes in human macrophage cells
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DOI：
10.1016/j.biomaterials.2009.04.019
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发表时间：
2009-09-01
期刊：
影响因子：
14
通讯作者：
Porter, Alexandra E.
中科院分区：
文献类型：
--
作者：
Cheng, Crystal;Muller, Karin H.;Porter, Alexandra E.
多壁碳纳米管（MWNTs）已被提出用于许多应用中，并且对它们对人类健康的潜在影响的关注导致了对理解MWNTs和人类细胞之间的相互作用的兴趣。一个重要的技术是可视化的细胞内分布的MWNT。我们将人巨噬细胞暴露于未纯化的多壁碳纳米管，发现细胞活力的降低与多壁碳纳米管的吸收有关，主要是由于坏死。用纯化的MWNTs和主要污染物Fe 2 O3本身处理的细胞仅从纳米管而不是从Fe 2 O3产生毒性。我们使用3-D暗场扫描透射电子显微镜（DF-STEM）断层扫描的冻干全细胞以及共聚焦和扫描电子显微镜（SEM）的图像的细胞摄取和分布的未纯化的多壁碳纳米管。我们观察到未纯化的MWNTs主动和被动地进入细胞，经常通过质膜插入细胞质和细胞核。这些表明，MWNTs可能会导致不完全的吞噬作用或机械穿透质膜，导致氧化应激和细胞死亡。(C)2009年由Elsevier Ltd.出版
Multi-walled carbon nanotubes (MWNTs) have been proposed for use in many applications and concerns about their potential effect on human health have led to the interest in understanding the interactions between MWNTs and human cells. One important technique is the visualisation of the intracellular distribution of MWNTs. We exposed human macrophage cells to unpurified MWNTs and found that a decrease in cell viability was correlated with uptake of MWNTs due to mainly necrosis. Cells treated with purified MWNTs and the main contaminant Fe2O3 itself yielded toxicity only from the nanotubes and not from the Fe2O3. We used 3-D dark-field scanning transmission electron microscopy (DF-STEM) tomography of freeze-dried whole cells as well as confocal and scanning electron microscopy (SEM) to image the cellular uptake and distribution of unpurified MWNTs. We observed that unpurified MWNTs entered the cell both actively and passively frequently insetting through the plasma membrane into the cytoplasm and the nucleus. These suggest that MWNTs may cause incomplete phagocytosis or mechanically pierce through the plasma membrane and result in oxidative stress and cell death. (C) 2009 Published by Elsevier Ltd.