Dinucleoside Phosphates Containing Arabinose or Deoxyxylose. Hydrolysis by Exonucleases and Stacking Properties
Dinucleoside Phosphates Containing Arabinose or Deoxyxylose. Hydrolysis by Exonucleases and Stacking Properties
复制标题
含有阿拉伯糖或脱氧木糖的二核苷磷酸。
DOI：
10.1080/07328319008045184
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Z. Shabarova
中科院分区：
文献类型：
--
作者：
N. Sokolova;N. Dolinnaya;N. F. Krynetskaya;Z. Shabarova
摘要合成了两对含有1-β-D-阿拉伯呋喃糖基)尿嘧啶(阿拉伯-U，Au)和1-β-3-D-2-脱氧-苏基-呋喃核糖)胸腺嘧啶(2‘-脱氧基-T，dxT)的二核苷单磷酸异构体。研究了蛇毒和脾脏磷酸二酯酶(PDE)对这些二聚体的降解动力学。此外，还用圆二色谱研究了它们的堆积行为。发现酶的切割速率与二聚体的构象之间存在相关性。研究发现，5‘端核苷残基的C-2’或C-3‘位构型变化，尤其是3’-羟基参与3‘-5’-磷酸二酯键形成的3‘-羟基的反转是关键。
Abstract Two pairs of isomeric dinucleoside monophosphates containing either 1-β-D-arabinofuranosyl)uracil (arabino-U, aU) or 1-β3-D-2-deoxy-threo-pentofuranosyl) thymine (2′-deoxyxylo-T, dxT) were synthesized. The kinetics of the hydrolysis of these dimers by venom and spleen phosphodiesterases (PDE) were studied. In addition, circular dichroism was used to study their stacking behaviour. A correlation was found to exist between the enzymatic cleavage rate and the conformation of the dimers. It was found that the configuration change at C-2′ or C-3′ in the 5′-terminal nucleoside residue, especially the inversion of 3′-hydroxyl involved into the internucleotide 3′-5′-phosphodiester linkage formation, was critical.