Molecular mechanisms of ceramide-mediated CD95 clustering
Molecular mechanisms of ceramide-mediated CD95 clustering
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DOI：
10.1006/bbrc.2001.5045
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发表时间：
2001-06-22
影响因子：
3.1
通讯作者：
Gulbins, E
中科院分区：
文献类型：
--
作者：
Grassmé, H;Schwarz, H;Gulbins, E
受体聚集已被认为是启动受体信号传导的重要机制。在此，我们发现富含鞘脂的膜筏中的神经酰胺介导了CD95的聚集。中和表面神经酰胺或抑制其内源性产生阻止了CD95聚集。此外，神经酰胺在细胞表面的应用触发了活性而非活性CD95的聚集。细胞凋亡的抑制通过中和表面神经酰胺或抑制神经酰胺释放在体外和体内。因此，我们得出结论，表面神经酰胺介导的CD95聚类，这是需要启动凋亡，至少在某些细胞类型。(C)中国科学出版社.
Receptor clustering has been suggested as a crucial mechanism to initiate receptor signaling Here we show that ceramide in sphingolipid-rich membrane rafts mediates clustering of CD95. Neutralization of surface ceramide or inhibition of its endogenous generation prevented CD95 clustering. Furthermore, application of ceramide at the cell surface triggered clustering of active but not inactive CD95. Apoptosis was inhibited by neutralization of surface ceramide or inhibition of ceramide release in vitro and in vivo. Thus, we conclude that surface ceramide mediates CD95 clustering, which is required for initiation of apoptosis, at least in some cell types. (C) zool Academic Press.