MiR-107 down-regulates SIAH1 expression in human breast cancer cells and silencing of miR-107 inhibits tumor growth in a nude mouse model of triple-negative breast cancer
MiR-107 down-regulates SIAH1 expression in human breast cancer cells and silencing of miR-107 inhibits tumor growth in a nude mouse model of triple-negative breast cancer
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DOI：
10.1002/mc.22320
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发表时间：
2016-05-01
影响因子：
4.6
通讯作者：
Song, Min
中科院分区：
文献类型：
--
作者：
Zhang, Li;Ma, Ping;Song, Min
我们已经报道了SIAH 1在人乳腺癌中下调并与细胞凋亡和侵袭相关。然而，导致SIAH 1下调的分子机制仍有待阐明。在这里，我们证明了miR-107直接下调人乳腺癌细胞中SIAH 1的表达。过表达miR-107可降低SIAH 1的表达，促进人乳腺癌细胞增殖、集落形成、迁移和侵袭，抑制凋亡。相反，miR-107的沉默增加了SIAH 1的表达，并抑制了MDA-MB-231细胞（一种三阴性乳腺癌（TNBC）细胞）的肿瘤生长。我们的研究结果表明，miR-107是人乳腺癌细胞中SIAH 1下调的上游调节因子，miR-107为治疗TNBC提供了潜在的有效靶点。(c)2015年威利期刊公司
We have reported that SIAH1 is down-regulated and associated with apoptosis and invasion in human breast cancer. However, the molecular mechanisms leading to SIAH1 down-regulation remain to be elucidated. Here, we demonstrated that miR-107 directly down-regulates SIAH1 expression in human breast cancer cells. Over- expression of miR-107 reduced SIAH1 expression, promoted human breast cancer cell proliferation, colony formation, migration and invasion, and inhibited apoptosis. On the contrary, silencing of miR-107 increased SIAH1 expression and inhibited the tumor growth of MDA-MB-231 cells, a kind of triple-negative breast cancer (TNBC) cells, in vitro and in vivo. Our results reveal that miR-107 is an upstream regulator for SIAH1 down-regulation in human breast cancer cells and miR-107 provides a potential effective target for the treatment of TNBC. (c) 2015 Wiley Periodicals, Inc.