Design and Implementation of a Wireless In-Ovo EEG/EMG Recorder
Design and Implementation of a Wireless In-Ovo EEG/EMG Recorder
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DOI：
10.1109/tbcas.2013.2251343
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发表时间：
2013-12-01
影响因子：
5.1
通讯作者：
Pompeiano, Maria
中科院分区：
文献类型：
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作者：
Di Pascoli, Stefano;Puntin, Daniele;Pompeiano, Maria
高等脊椎动物（鸟类和哺乳动物）睡眠和大脑活动节律的发育起源目前尚不清楚。为了建立一个实验系统，其中这些可以更好地阐明，我们设计，建造和测试的系统，用于记录脑电和肌电信号在卵内从鸡胚孵育16-21天。该系统可以在孵化过程中保持附着在个体受试者上，并在出生后继续佩戴。通过外科手术植入胚胎头部的电极线连接到一个电池驱动的超便携式发射器，该发射器可以连接到蛋壳上，也可以佩戴在背部。该变送器最多可处理6个通道的数据，最大采样频率为500 Hz，分辨率为12位。无线电链路使用4兆赫的载波频率，最大传输速率为500千比特/秒;生产了兼容蛋内记录和自由移动鸟类出生后记录的接收天线。与PC的一个USB端口连接的接收器传输数据以进行数字存储。该系统基于分立的现成组件，可使用单个锂纽扣电池连续运行数天，本底噪声水平为0.35 mu V。发射机尺寸为16 x 13 x 1.5 mm，不含电池时重量为0.7 g。该微处理器允许灵活的操作模式，而这些模式通常在通过自组织混合信号芯片实现的其他小型多通道采集系统中不可用。
The developmental origins of sleep and brain activity rhythms in higher vertebrate animals (birds and mammals) are currently unknown. In order to create an experimental system in which these could be better elucidated, we designed, built and tested a system for recording EEG and EMG signals in-ovo from chicken embryos incubated for 16-21 days. This system can remain attached to the individual subject through the process of hatching and continue to be worn post-natally. Electrode wires surgically implanted on the head of the embryo are connected to a battery-operated ultraportable transmitter which can either be attached to the eggshell or worn on the back. The transmitter processes up to 6 channels of data with a maximum sampling frequency of 500 Hz and a resolution of 12 bits. The radio link uses a carrier frequency of 4 MHz, and has a maximum transfer rate of 500 kbit/s; receiving antennas compatible with both in-egg recordings and post-natal recordings from freely-moving birds were produced. A receiver connected with one USB port of a PC transmits the data for digital storage. This system is based on discrete, off-the-shelf components, can provide a few days of continuous operation with a single lithium coin battery, and has a noise floor level of 0.35 mu V. The transmitter dimensions are 16 x 13 x 1.5 mm and the weight without the battery is 0.7 g. The microprocessor allows flexible operation modes not usually made available in other small multichannel acquisition systems implemented by means of ad hoc mixed signal chips.