EXTENSION OF THE LINKAGE MAP IN CITRUS USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS AND RFLP MAPPING OF COLD-ACCLIMATION-RESPONSIVE LOCI
EXTENSION OF THE LINKAGE MAP IN CITRUS USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS AND RFLP MAPPING OF COLD-ACCLIMATION-RESPONSIVE LOCI
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DOI：
10.1007/bf00222455
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发表时间：
1994-11-01
影响因子：
5.4
通讯作者：
MOORE, GA
中科院分区：
文献类型：
--
作者：
CAI, Q;GUY, CL;MOORE, GA
柑橘中已开始利用随机扩增多态性DNA（RAPD）标记进行遗传图谱构建。在测试的140个随机引物中，约有一半能获得可重复的扩增DNA片段多态性，揭示出266个分离位点。利用柚[Citrus grandis (L.) Osb.]×[柚×枳壳（Poncirus trifoliata (L.) Raf.）]的60个回交一代（BC1）子代对这些位点进行连锁检测。构建了一个核心连锁图谱，它由9个连锁群组成，包含109个RAPD标记以及51个先前定位的限制性片段长度多态性（RFLP）和同工酶标记。另外还有79个由于其等位基因组成而无法明确排序的标记与各个连锁群相关联，并相对于核心图谱进行了展示。核心图谱总长1192厘摩（cM），位点间平均距离为7.5厘摩，估计覆盖了基因组的70 - 80%。具有偏分离模式的位点聚集在几个连锁群上。各个位点簇在等位基因组成上偏向于一方亲本，通常是柚。这个相对饱和的连锁图谱最终将用于鉴定柑橘中耐寒和耐盐的数量性状位点。作为开端，我们已经对一个冷驯化响应的互补DNA（cDNA）所检测到的三个位点进行了定位。
Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of Citrus grandis (L.) Osb. x [Citrus grandis (L.) Osb. x Poncirus trifoliata (L.) Raf.]. A core linkage map was constructed that consists of nine linkage groups containing 109 RAPD markers and 51 previously-mapped RFLP and isozyme markers. A further 79 markers that could not be ordered unambiguously because of their allelic constitution were associated with individual linkage groups and are shown in relation to the core map. The core map has a total length of 1192 cM with an average distance of 7.5 cM between loci and is estimated to cover 70-80% of the genome. Loci with distorted segregation patterns clustered on several linkage groups. Individual clusters of loci were skewed in allelic composition toward one or the other parent, usually C. grandis. This relatively-saturated linkage map will eventually be used to identify quantitative trait loci for cold and salt-tolerance in Citrus. As a beginning we have mapped three loci detected by a cold-acclimation-responsive cDNA.