Dental monomers inhibit LPS-induced cytokine release from the macrophage cell line RAW264.7
Dental monomers inhibit LPS-induced cytokine release from the macrophage cell line RAW264.7
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DOI：
10.1016/j.toxlet.2012.11.010
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发表时间：
2013-02-04
影响因子：
3.5
通讯作者：
Mathisen, Gro Haarklou
中科院分区：
文献类型：
--
作者：
Bolling, Anette Kocbach;Samuelsen, Jan Tore;Mathisen, Gro Haarklou
甲基丙烯酸酯单体已经在新鲜固化的牙科填充物的水提取物中被鉴定。目前测试的假设是低浓度的三甘醇二甲基丙烯酸酯（TEGDMA）和甲基丙烯酸2-羟乙酯（HEMA）单独或组合干扰LPS诱导的细胞因子从巨噬细胞系RAW 264.7释放。将细胞暴露于5-200 μ M的单体24小时，然后组合暴露于单体和LPS 24小时。TEGDMA减少了LPS诱导的白细胞介素-1 β（IL-1 β）和肿瘤坏死因子-α（TNF-α）的释放，而HEMA仅减少了IL-1 β的释放。共暴露于两种单体表明累加效应。此外，减少细胞因子释放持续24小时后终止的单体曝光。LPS诱导的转录前信号通路中蛋白质（CD 14、p-ERK 1/2、p-p38、p-JNK、p-I kappa B-alpha和p-NF kappa B-p65）的活化未因单体暴露而改变，IL-1 β和TNF-α mRNA水平也未改变。然而，单体处理降低了LPS诱导的pro-IL-1 β水平。因此，HEMA和TEGDMA可能干扰这些细胞因子的合成和释放的转录后调节。总体而言，结果表明，低浓度的单体可能会导致受损的巨噬细胞反应，这些影响可以持续长达24小时后曝光。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Methacrylate monomers have been identified in aqueous extracts of freshly cured dental fillings. The hypothesis tested presently was that low concentrations of triethyleneglycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA) alone or in combination interfere with the LPS-induced release of cytokines from the macrophage cell line RAW264.7. The cells were exposed to 5-200 mu M of monomers for 24 h followed by a 24 h combined exposure to monomers and LPS. TEGDMA reduced LPS-induced release of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha), whereas HEMA only reduced IL-1 beta release. Co-exposure to the two monomers indicated an additive effect. Moreover, the reduced cytokine release persisted for 24 h after termination of the monomer exposure. The LPS-induced activation of proteins in pre-transcriptional signaling pathways (CD14, p-ERK1/2, p-p38, p-JNK, p-I kappa B-alpha and p-NF kappa B-p65) was not altered by monomer exposure, neither were the levels of IL-1 beta and TNF-alpha mRNA. However, the LPS-induced level of pro-IL-1 beta was decreased by the monomer treatment. Thus, HEMA and TEGDMA may interfere with post-transcriptional regulation of synthesis and release of these cytokines. Overall, the results suggest that low concentrations of monomers may cause impaired macrophage responses, and that these effects can persist for up to 24 h after exposure. (C) 2012 Elsevier Ireland Ltd. All rights reserved.