PEPTIDE NUCLEIC-ACIDS AND THEIR POTENTIAL APPLICATIONS IN BIOTECHNOLOGY
PEPTIDE NUCLEIC-ACIDS AND THEIR POTENTIAL APPLICATIONS IN BIOTECHNOLOGY
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DOI：
10.1016/0167-7799(93)90097-s
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发表时间：
1993-09-01
影响因子：
17.3
通讯作者：
NIELSEN, PE
中科院分区：
文献类型：
--
作者：
BUCHARDT, O;EGHOLM, M;NIELSEN, PE
肽核酸(PNA)是一种新型的DNA模拟物，它的糖-磷酸骨架被基于氨基酸1-3的骨架所取代。PNA与DNA和RNA的序列特异性结合比未修饰的DNA具有更高的亲和力和特异性。它们能抵抗血清和细胞提取物中的核酸酶和蛋白水解酶的攻击，因此，作为诊断和生物分子探针，以及作为反义和抗基因药物，似乎非常有希望。
Peptide nucleic acids (PNAs) are novel DNA mimics in which the sugar-phosphate backbone has been replaced with a backbone based on amino acids1-3. PNAs exhibit sequence-specific binding to DNA and RNA with higher affinities and specificities than unmodified DNA. They,are resistant to nuclease and protease attack in serum and cellular extracts and, thus, appear very promising as diagnostic and biomolecular probes, and possibly as antisense and antigene drugs.