Effects of CeO2 nanoparticles on microbial metabolism
Effects of CeO2 nanoparticles on microbial metabolism
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DOI：
10.1016/j.chemgeo.2014.10.026
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发表时间：
2015-01
期刊：
影响因子：
3.9
通讯作者：
Shota Masaki;Hiroyuki Shiotsu;T. Ohnuki;F. Sakamoto;S. Utsunomiya
中科院分区：
文献类型：
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作者：
Shota Masaki;Hiroyuki Shiotsu;T. Ohnuki;F. Sakamoto;S. Utsunomiya
为了了解纳米颗粒对微生物的影响，我们实验研究了CeO2纳米颗粒（CeNPs）对酵母菌（S. cerevisiae），专注于微生物代谢物和细胞内蛋白质。从含有0、10、100和250 ppm CeNP的酵母提取物蛋白胨葡萄糖培养基中收获酵母，并在三种不同pH值：3、5和7的ImM NaCl溶液中孵育120小时。酵母释放有机物质，P，K，和Mg到NaCl溶液中在所有pH值，即使没有CeNPs。在暴露于CeNPs后，在释放的有机物质和细胞内蛋白质中检测到明显的差异。高效液相色谱显示，从酵母释放的各种有机物种的表达或抑制后，暴露于CeNPs。虽然细胞毒性不是由CeNPs引起的，但细胞内蛋白质的肽质量指纹分析的结果显示，在暴露于CeNPs后，糖酵解酶Eno2p表达。这些结果表明，纳米颗粒有可能改变微生物的代谢，导致周围环境中释放物质的组成发生变化。
To understand the effects of nanoparticles on microorganisms, we experimentally investigated the effects of CeO2nanoparticles (CeNPs) on yeast (S. cerevisiae) focusing on microbial metabolites and intracellular proteins. The yeast were harvested from a yeast extract peptone dextrose medium containing 0, 10, 100, and 250 ppm of CeNPs and incubated for 120 h in 1 mM NaCl solution at three different pH values: 3, 5, and 7. The yeast released organic matter, P, K, and Mg into the NaCl solution at all pH values, even without CeNPs. Distinct differences were detected in the released organic species and intracellular proteins after exposure to CeNPs. High-performance liquid chromatography revealed that various organic species released from the yeast were expressed or suppressed after exposure to CeNPs. Although cytotoxicity was not caused by CeNPs, the results of the peptide mass fingerprint analysis of the intracellular protein revealed that Eno2p, a glycolysis enzyme, was expressed after exposure to CeNPs. These results suggest that nanoparticles have the potential to alter microbial metabolism, leading to changes in the compositions of the released substances in the surrounding environment.