Effects of chronic treatment with new strains of Lactobacillus plantarumon cognitive, anxiety- and depressive-like behaviors in male mice
Effects of chronic treatment with new strains of Lactobacillus plantarumon cognitive, anxiety- and depressive-like behaviors in male mice
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DOI：
10.1371/journal.pone.0234037
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发表时间：
2020-06-19
期刊：
影响因子：
3.7
通讯作者：
Villela Marinho, Eduardo Ary
中科院分区：
文献类型：
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作者：
Barros-Santos, Thaisa;Oliveira Silva, Kallyane Santos;Villela Marinho, Eduardo Ary
精神益生菌对应于一类益生菌，主要属于乳酸杆菌和双歧杆菌属，能够产生神经活性物质，如γ -氨基丁酸（GABA）和血清素，它们对脑-肠轴起作用。有证据表明，精神生物制剂可以对情绪、焦虑和认知产生有益的影响。本研究评估了两种植物乳杆菌新菌株的慢性给药效果。plantarum286 （Lp 286）和l。plantarum81 （Lp 81）分别从可可（Theobroma cacaoL.）和cupuacu （Theobroma triflorum）发酵中分离出来，对雄性瑞士小鼠的认知、焦虑和抑郁样行为有影响。各组动物分别灌胃0.85%生理盐水加15%脱脂牛奶的培养液、Lp 286 （10(9)/0.1 ml CFU）或Lp 81 (10(9)/0.1 ml CFU)，持续30天，测试动物的一般运动活动、强迫游泳测试中的抑郁样行为、加迷宫区分回避任务中的学习/记忆和焦虑样行为。Lp 286和Lp 81菌株对运动活动和学习记忆没有影响。在我们的实验条件下，lp286菌株具有抗抑郁和抗焦虑的作用。我们的发现增加了现有的证据，表明乳酸菌属的益生菌可能发挥精神生物潜能，并引入了一种新的菌株，lp286，作为预防或治疗精神障碍的潜在候选菌株。
Psychobiotics correspond to a class of probiotics, mainly of the genusLactobacillusandBifidobacterium, capable of producing neuroactive substances, such as gamma-aminobutyric acid (GABA) and serotonin, which exert effects on the brain-gut axis. Evidence suggests that psychobiotics can have a beneficial effect on mood, anxiety and cognition. The present study evaluated the effects of chronic administration of two new strains ofLactobacillus plantarum,L.plantarum286 (Lp 286) andL.plantarum81 (Lp 81) isolated from the fermentation of cocoa (Theobroma cacaoL.) and cupuacu (Theobroma grandiflorum), respectively, on cognitive, anxiety- and depressive-like behaviors in male Swiss mice. Different groups of animals were administered (oral gavage) solutions of vehicle (0.85% saline plus 15% skim milk), Lp 286 (10(9)/0.1 ml CFU) or Lp 81 (10(9)/0.1 ml CFU) for 30 days, and animals were tested for general locomotor activity, depressive-like behavior in the forced swim test, and learning/memory and anxiety-like behavior in the plus-maze discriminative avoidance task. Treatment with the strains Lp 286 and Lp 81 did not interfere with locomotor activity or learning and memory. The Lp 286 strain exerted anti-depressant- and anxiolytic-like effects under our experimental conditions. Our findings add to the current body of evidence suggesting that probiotics from the genusLactobacillusmay exert psychobiotic potential and introduce a new strain, Lp 286, as a potential candidate in the prevention or as therapeutic adjuvant in the treatment of mental disorders.