Quenching Efficiency of Quantum Dots Conjugated to Lipid Bilayers on Graphene Oxide Evaluated by Fluorescence Single Particle Tracking
Quenching Efficiency of Quantum Dots Conjugated to Lipid Bilayers on Graphene Oxide Evaluated by Fluorescence Single Particle Tracking
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DOI：
10.3390/app12083733
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发表时间：
2022-04
期刊：
影响因子：
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通讯作者：
Y. Okamoto;S. Iwasa;R. Tero
中科院分区：
文献类型：
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作者：
Y. Okamoto;S. Iwasa;R. Tero
在氧化石墨烯（GO）上形成的脂质双层上进行量子点（QD）的单粒子观察。GO的长程荧光猝灭已被应用于各种生物分子的生物传感。在这项研究中，我们展示了单粒子观察的量子点支持的脂质双层，旨在检测脂质和蛋白质分子的淬灭效率的脂质双层荧光单粒子跟踪（SPT）。通过囊泡融合方法在沉积于热氧化硅基底上的GO薄片上形成单脂质双层或双脂质双层。将量子点偶联到脂质双层上，在GO的猝灭作用下获得量子点的单粒子图像。从具有和不具有GO的区域上的荧光强度评估单个QD的猝灭效率。得到了反映脂质双层层数的猝灭效率。
A single particle observation of quantum dots (QDs) was performed on lipid bilayers formed on graphene oxide (GO). The long-range fluorescence quenching of GO has been applied to biosensing for various biomolecules. We demonstrated the single particle observation of a QD on supported lipid bilayers in this study, aiming to detect the quenching efficiency of lipid and protein molecules in a lipid bilayer by fluorescence single particle tacking (SPT). A single lipid bilayer or double lipid bilayers were formed on GO flakes deposited on a thermally oxidized silicon substrate by the vesicle fusion method. The QDs were conjugated on the lipid bilayers, and single particle images of the QDs were obtained under the quenching effect of GO. The quenching efficiency of a single QD was evaluated from the fluorescence intensities on the regions with and without GO. The quenching efficiency reflecting the layer numbers of the lipid bilayers was obtained.