Assembling Defined DNA Nanostructure with Nitrogen-Enriched Carbon Dots for Theranostic Cancer Applications
Assembling Defined DNA Nanostructure with Nitrogen-Enriched Carbon Dots for Theranostic Cancer Applications
复制标题
用富氮碳点组装确定的 DNA 纳米结构，用于癌症治疗应用
DOI：
10.1002/smll.201906975
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发表时间：
2020-05-01
期刊：
影响因子：
13.3
通讯作者：
Qian, Hang
中科院分区：
文献类型：
--
作者：
Wu, Di;Li, Bang Lin;Qian, Hang
DNA纳米结构作为药物输送、生物传感和生物成像的支架，由于其在生理环境中的脆弱性而受到阻碍，不利于结合任意客体分子和其他理想的功能。DNA纳米结构与小分子在另一种体系中的非正则自组装是扩展其在多学科领域中应用的一种有吸引力的策略，但很少有人探索。这项工作报告了一种富氮碳点(NCDS)介导的DNA纳米结构自组装策略。由于NCDS具有良好的光致发光和光动力学性质，所获得的DNA/NCDS纳米复合材料在生物成像和抗癌治疗方面具有巨大的潜力。NCDs可以在较大的温度和pH范围内无镁等温地介导DNA纳米颗粒(NPNCD)的自组装。为了探索NPNCD在生物医学中的潜在应用，对NPNCD的细胞毒性和细胞摄取效率进行了评价。NPNCD与KRAS siRNA((NPK)-K-NCD)进一步偶联用于KRAS突变的非小细胞肺癌的治疗。(NPK)-K-NCD具有良好的基因敲除效率和体外抗癌作用。目前的研究表明，将NCDs与可编程DNA纳米结构结合是赋予DNA纳米结构新功能的有效策略，NPNCD可能是一种潜在的理论平台，具有进一步微调CDS的近红光荧光或光热活性等性质。
DNA nanostructures as scaffolds for drug delivery, biosensing, and bioimaging are hindered by its vulnerability in physiological settings, less favorable of incorporating arbitrary guest molecules and other desirable functionalities. Noncanonical self-assembly of DNA nanostructures with small molecules in an alternative system is an attractive strategy to expand their applications in multidisciplinary fields and is rarely explored. This work reports a nitrogen-enriched carbon dots (NCDs)-mediated DNA nanostructure self-assembly strategy. Given the excellent photoluminescence and photodynamic properties of NCDs, the obtained DNA/NCDs nanocomplex holds great potential for bioimaging and anticancer therapy. NCDs can mediate DNA nanoprism (NPNCD) self-assembly isothermally at a large temperature and pH range in a magnesium-free manner. To explore the suitability of NPNCD in potential biomedical applications, the cytotoxicity and cellular uptake efficiency of NPNCD are evaluated. NPNCD with KRAS siRNA ((NPK)-K-NCD) is further conjugated for KRAS-mutated nonsmall cell lung cancer therapy. The (NPK)-K-NCD shows excellent gene knockdown efficiency and anticancer effect in vitro. The current study suggests that conjugating NCDs with programmable DNA nanostructures is a powerful strategy to endow DNA nanostructures with new functionalities, and NPNCD may be a potential theranostic platform with further fine-tuned properties of CDs such as near-red fluorescence or photothermal activities.