Ocean acidification and pathogen exposure modulate the immune response of the edible mussel Mytilus chilensis
Ocean acidification and pathogen exposure modulate the immune response of the edible mussel Mytilus chilensis
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DOI：
10.1016/j.fsi.2017.08.047
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发表时间：
2017-11-01
影响因子：
4.7
通讯作者：
Detree, Camille
中科院分区：
文献类型：
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作者：
Castillo, Nicole;Saavedra, Luisa M.;Detree, Camille
海洋酸化（OA）是大气二氧化碳（CO2）增加的主要后果之一，影响海洋生物的关键生物过程，如发育、生长和免疫反应。然而，关于OA对海洋无脊椎动物应对病原体能力的影响的研究很少。本研究探讨了OA和细菌感染单独和联合作用对紫贻贝抗氧化系统、抗菌肽和模式识别受体相关基因转录表达的影响。M的个体。智利人暴露在两种浓度的pCO（2）（550和1200 μ atm）下60天，分别代表OA的当前和未来情景，然后注射致病细菌鳗弧菌。结果表明，OA暴露后，C型凝集素和Mytilin B的上调和Mytilin A和PGRP的下调的免疫调节。在用鳗弧菌攻击后，这种免疫调节模式被部分抵消，C型凝集素和Mytilin B下调，Mytilin A上调。反过来，这些结果证明pCO（2）驱动的OA可能会在感染的早期阶段触发特定的免疫相关基因，促进抗菌肽和模式识别受体的转录。这项研究提供了新的证据，说明海洋中较高的CO2条件如何调节双壳类的免疫反应，以及海洋种群对全球变化情景的适应力的一个因素。(C)2017爱思唯尔有限公司版权所有
Ocean acidification (OA) is one of the main consequences of increasing atmospheric carbon dioxide (CO2), impacting key biological processes of marine organisms such as development, growth and immune response. However, there are scarce studies on the influence of OA on marine invertebrates' ability to cope with pathogens. This study evaluated the single and combined effects of OA and bacterial infection on the transcription expression of genes related to antioxidant system, antimicrobial peptides and pattern recognition receptors in the edible mussel Mytilus chilensis. Individuals of M. chilensis were exposed during 60 days at two concentrations of pCO(2) (550 and 1200 mu atm) representing respectively current and future scenario of OA and were then injected with the pathogenic bacterium Vibrio anguillarum. Results evidenced an immunomodulation following the OA exposure with an up-regulation of C-type Lectin and Mytilin B and a down-regulation of Myticin A and PGRP. This immunomodulation pattern is partially counteracted after challenge with V. anguillarum with a down-regulation of the C-type lectin and Mytilin B and the up-regulation of Myticin A. In turn, these results evidence that pCO(2)-driven OA scenarios might triggers specific immune-related genes at early stages of infection, promoting the transcription of antimicrobial peptides and patterns recognition receptors. This study provides new evidence of how the immune response of bivalves is modulated by higher CO2 conditions in the ocean, as well one factor for the resilience of marine population upon global change scenarios. (C) 2017 Elsevier Ltd. All rights reserved.