HEPATOCYTE GROWTH-FACTOR STIMULATES GROWTH OF HEMATOPOIETIC PROGENITOR CELLS
HEPATOCYTE GROWTH-FACTOR STIMULATES GROWTH OF HEMATOPOIETIC PROGENITOR CELLS
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DOI：
10.1006/bbrc.1993.1801
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发表时间：
1993-07-15
影响因子：
3.1
通讯作者：
NAKAMURA, T
中科院分区：
文献类型：
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作者：
MIZUNO, K;HIGUCHI, O;NAKAMURA, T
肝细胞生长因子（HGF）是一种间充质来源的多肽，能刺激各种类型细胞的生长、运动和形态发生，主要是上皮细胞。我们现在已经确定了HGF的另一种生物活性；这个因素可能在早期造血中起作用。我们检测了HGF对多种小鼠造血祖肿瘤细胞系生长的影响，发现HGF刺激髓系白血病细胞系NFS-60的DNA合成。HGF对nf -60细胞的促有丝分裂作用与白细胞介素3 （IL-3）的作用呈叠加作用。另一方面，HGF对其他髓系白血病细胞系如DA-3和FDC-P1细胞无明显影响。[125I]HGF特异性结合的Scatchard分析显示，在NFS-60和DA-3细胞上表达了高亲和力的HGF受体，但在FDC-P1细胞上没有表达。c- metmrna的表达与高亲和力HGF受体的存在密切相关。由于使用的髓系白血病细胞系是处于髓系分化早期的细胞，HGF可能在早期造血中发挥作用。
Hepatocyte growth factor (HGF), a mesenchymal-derived polypeptide, stimulates growth, motility and morphogenesis of various types of cells, most predominantly of epithelial cells. We have now identified an additional biological activity of HGF; this factor probably has a role in early hematopoiesis. We examined the effects of HGF on the growth of various murine hematopoietic progenitor tumor cell lines and found that HGF stimulated DNA synthesis in the myeloid leukemia cell line, NFS-60. The mitogenic effect of HGF on NFS-60 cells was additive with the effect of interleukin 3 (IL-3). On the other hand, HGF had no apparent effect on other myeloid leukemia cell lines examined, such as DA-3 and FDC-P1 cells. Scatchard analysis of specific binding of [125I]HGF revealed expression of a high-affinity HGF receptor on NFS-60 and DA-3 cells, but not on FDC-P1 cells. Expression ofc-metmRNA correlated well with the existence of a high-affinity HGF receptor. Since the myeloid leukemia cell lines used are cells in the early stage of myeloid differentiation, HGF may play a role in early hematopoiesis.