Protective efficacy of Clinacanthus nutans on yellow-head disease in black tiger shrimp (Penaeus monodon)
Protective efficacy of Clinacanthus nutans on yellow-head disease in black tiger shrimp (Penaeus monodon)
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DOI：
10.3147/jsfp.33.401
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发表时间：
1998-10-01
期刊：
影响因子：
0.6
通讯作者：
Yoshimizu, M
中科院分区：
文献类型：
--
作者：
Direkbusarakom, S;Ruangpan, L;Yoshimizu, M
以泰国著名的抗病毒中药垂穗吊兰（Clinacanthus nutans Lindua）的叶片为原料，采用索氏提取法，用乙醇提取抗病毒活性物质。对提取物进行了抗斑节对虾黄头弹状病毒（YRV）的活性测试。通过观察注射提取物处理的病毒的虾的死亡率来研究杀病毒效果。结果表明，C. nutans在体外抑制YRV，最低浓度为1 μ g/ml。通过口服该提取物混合颗粒来测试该提取物对对虾YRV感染的保护效力。结果表明，以0.1、1和10 g/kg颗粒饲料投喂的对虾保护率分别为44.6%、57.4%和4.2%。以1 g/kg的槟榔提取物与颗粒剂混合使用对对虾YRV感染的控制效果最好。
The leaves of Clinacanthus nutans Lindua, a well-known Thai traditional medicine against viral disease in human being, were used to extract antiviral substances by ethanol using soxhlet apparatus. The extract was tested for its activity against yellow-head rhabdovirus (YRV) in black tiger shrimp (Penaeus monodon). Virucidal effect was investigated by observation of mortality of the shrimp injected with the extract-treated virus. The results showed that extract of C. nutans inhibited YRV in vitro with the minimum concentration of 1 mu g/ml. Protective efficacy of the extract on YRV infection in shrimp was tested by oral administration of the extract mixed pellet. As the result, the protective efficacies obtained were 44.6, 57.4 and 4.2% in the shrimp groups fed the extract at 0.1, 1 and 10 g/kg pellet, respectively, indicating that the C. nutans extract mixed with pellet at 1 g/kg could most effectively control YRV infection in shrimp.