Amelioration of excess collagen IαI, fibrosis, and smooth muscle growth in TNBS-induced colitis in IGF-I(+/-) mice.
Amelioration of excess collagen IαI, fibrosis, and smooth muscle growth in TNBS-induced colitis in IGF-I(+/-) mice.
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DOI：
10.1002/ibd.21437
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发表时间：
2011-03
影响因子：
4.9
通讯作者：
Kuemmerle, John F.
中科院分区：
文献类型：
--
作者：
Mahavadi, Sunila;Flynn, Robert S.;Grider, John R.;Qiao, Li-Ya;Murthy, Karnam S.;Hazelgrove, Krystina B.;Kuemmerle, John F.
Strictures occur in ~30% of patients with Crohn’s disease and are characterized by intestinal smooth muscle hyperplasia, hypertrophy, and fibrosis due to excess extracellular matrix production including collagen. Insulin-like Growth Factor-I (IGF-I) expression is increased in smooth muscle cells of the muscularis propria in Crohn’s disease and in animal models of Crohn’s disease, including TNBS-induced colitis. While upregulated IGF-I is conjectured to cause smooth muscle cell growth and collagen production in the inflamed intestine, its role in the development of fibrosis has not been directly demonstrated. Colitis was induced in IGF-I(+/−) or wildtype C57BL/6J mice by rectal administration of TNBS or ethanol vehicle. After 7 days colonic smooth muscle cells were isolated and used to prepare RNA or protein lysates. Transcript levels of IGF-IEa, IGF binding protein (IGFBP)-3, IGFBP-5, TGF-β1 and collagen IαI were measured by quantitative RT-PCR. Corresponding protein levels were measured by western blot or ELISA. Fibrosis was measured using digital image analysis of Masson’s-trichrome stained histologic sections. In IGF-I(+/−) mice, which express significantly lower levels of IGF-I than wildtype, the response to TNBS-induced colitis: upregulation of IGF-I, IGFBP-3, IGFBP-5, and collagen IαI expression, the resulting collagen deposition, and fibrosis are all significantly diminished compared to C57BL/6J wildtype controls. TGF-β1 expression and its increase following TNBS administration are not altered in IGF-I(+/−) mice compared to wildtype. The findings indicate IGF-I is a key regulator in intestinal smooth muscle of smooth hyperplasia and excess collagen production that leads to fibrosis and longterm to stricture formation.
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影响因子：
29.4
作者：
GRAHAM, MF;BRYSON, GR;DIEGELMANN, RF
通讯作者：
DIEGELMANN, RF
影响因子：
29.4
作者：
Ohneda, K;Ulshen, MH;Lund, PK
通讯作者：
Lund, PK
DOI：
10.1152/ajpgi.1998.275.2.g342
发表时间：
1998-08-01
影响因子：
4.5
作者：
Teng, BQ;Murthy, KS;Makhlouf, GM
通讯作者：
Makhlouf, GM
DOI：
10.1152/ajpgi.00310.2002
发表时间：
2003-03-01
影响因子：
4.5
作者：
Kuemmerle, JF
通讯作者：
Kuemmerle, JF
影响因子：
29.4
作者：
Kuemmerle, JF
通讯作者：
Kuemmerle, JF