DIFFERENT CELLULAR BACKGROUNDS CONFER A MARKED ADVANTAGE TO EITHER MUTANT OR WILD-TYPE MITOCHONDRIAL GENOMES
DIFFERENT CELLULAR BACKGROUNDS CONFER A MARKED ADVANTAGE TO EITHER MUTANT OR WILD-TYPE MITOCHONDRIAL GENOMES
复制标题
DOI：
10.1073/pnas.92.14.6562
复制
发表时间：
1995-07-03
影响因子：
11.1
通讯作者：
HOLT, IJ
中科院分区：
文献类型：
--
作者：
DUNBAR, DR;MOONIE, PA;HOLT, IJ
在将具有突变型和野生型线粒体DNA（mtDNA）的混合物的线粒体引入人rho degrees细胞系（143 B，206）后，Yoneda等人，[Yoneda，M.，Chomyn，A.，Martinuzzi，A.，Hurko，0和Attardi，G，（1992）Proc.Natl. Acad. Sci. USA 89，11164-11168]在许多胞质杂种克隆中观察到两种线粒体基因型比例的变化，在观察到变化的每种情况下，突变mtDNA的比例增加。（143 B，206 rho度），我们还产生了线粒体基因型稳定或突变mtDNA比例增加的胞质杂种。然而，在另一个rho degrees细胞系中相同突变mtDNA类型的时间分析揭示了一个相当不同的结果。这些结果表明，受体细胞的核遗传背景（rho度）可以影响细胞胞质杂种中突变型和野生型线粒体基因组的分离。
After the introduction of mitochondria with a mixture of mutant and wild-type mitochondrial DNA (mtDNA) into a human rho degrees cell line (143B,206), Yoneda ct al, [Yoneda, M., Chomyn, A., Martinuzzi, A., Hurko, O, and Attardi, G, (1992) Proc. Natl. Acad. Sci. USA 89, 11164-11168] observed a shift in the proportion of the two mitochondrial genotypes in a number of cybrid clones, In every case where a shift was observed, there was an increase in the proportion of mutant mtDNA, By using the same cell line (143B,206 rho degrees), we also generated cybrids that were either stable in their mitochondrial genotype or showed an increase in the proportion of mutant mtDNA. However, temporal analysis of the same mutant mtDNA type in another rho degrees cell line revealed a quite distinct outcome. Those clones that showed a change shifted toward higher levels of wild-type rather than mutant mtDNA, These results indicate that the nuclear genetic background of the recipient (rho degrees) cell can influence the segregation of mutant and wild-type mitochondrial genomes in cell cybrids.