UV-visible and infrared methods for investigating lipid-rhodopsin membrane interactions.
UV-visible and infrared methods for investigating lipid-rhodopsin membrane interactions.
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用于研究脂质-视紫红质膜相互作用的紫外-可见和红外方法。
DOI：
10.1007/978-1-62703-023-6_8
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Brown,MichaelF
中科院分区：
文献类型：
--
作者：
Brown,MichaelF
我们描述了用于表征膜双层环境中视紫红质的脂质-蛋白质相互作用的实验紫外可见和傅里叶变换红外（FTIR）光谱方法。结合红外光谱和紫外-可见差光谱技术，研究了视紫红质活化过程中结构和功能的变化。膜脂如何稳定各种视紫红质光产物的研究类似于在功能的获得或丧失方面使蛋白质突变。解释的结果需要一个灵活的表面模型，通过相关的生物活性的材料特性来解释膜脂质-蛋白质相互作用。
We describe experimental UV–visible and Fourier transform infrared (FTIR) spectroscopic methods for characterizing lipid–protein interactions for rhodopsin in a membrane bilayer environment. The combination of FTIR and UV–visible difference spectroscopy is used to monitor the structural and functional changes during rhodopsin activation. Investigations of how membrane lipids stabilize various rhodopsin photoproducts are analogous to mutating the protein in terms of gain or loss of function. Interpretation of the results entails a flexible surface model for explaining membrane lipid–protein interactions through material properties relevant to biological activity.