Forchlorfenuron, a phenylurea cytokinin, disturbs septin organization in Saccharomyces cerevisiae
Forchlorfenuron, a phenylurea cytokinin, disturbs septin organization in Saccharomyces cerevisiae
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DOI：
10.1266/ggs.79.199
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发表时间：
2004-08-01
影响因子：
1.1
通讯作者：
Toh-e, A
中科院分区：
文献类型：
--
作者：
Iwase, M;Okada, S;Toh-e, A
细胞分裂素，参与胞质分裂，已被确定在各种真菌和动物细胞。为了分析septin的功能，靶向septin的药物将是有用的;然而，迄今为止还没有这样的药物可用。我们偶然发现，合成的植物细胞分裂素氯吡脲（FCF，N-（2-氯-4-吡啶基）-N-苯基脲，4PU 300）对酿酒酵母胞质分裂有干扰作用。FCF处理后，芽颈处的Septin结构发生变形，芽颈外出现毒样Septin。在这些条件下，肌动蛋白的本地化是正常的和Gin 4，这是本地化在芽颈septin-dependent的方式，被发现保持在芽颈的位置显然是正常的septin，而它不是共本地化的变形septin在芽颈或septin外看到的芽颈。FCF给药立即诱导芽颈外产生零星的隔蛋白结构，这些结构在药物去除后迅速消失。这些结果表明，FCF可能是一种很有前途的研究septin结构和功能的药物。
Septins, which are involved in cytokinesis, have been identified in a variety of fungi and animal cells. For analysis of the function of septin, drugs targeting septin would be useful; however, no such drugs have been available hitherto. By serendipity, we found that forchlorfenuron (FCF, N-(2-chloro-4-pyridyl)-N-phenylurea, 4PU300), a synthetic plant cytokinin, disturbed cytokinesis in Saccharomyces cerevisiae. Upon administration of FCF, septin structures at the bud neck became deformed and filament-like septin appeared outside of the neck. Under these conditions, the localization of actin was normal and Gin4, which is localized at the bud neck in a septin-dependent manner, was found to remain at the location of apparently normal septin at the bud neck, whereas it was not co-localized to the deformed septin at the bud neck or to septin seen outside the bud neck. FCF administration immediately induced production of sporadic septin structures outside the bud neck, and these structures disappeared promptly upon removal of the drug. Taken together, these findings indicate that FCF maybe a promising drug for investigating the structure and function of septin.